Abstract ε-Caprolactone is a monomer of poly(ε-caprolactone) which has been widely used in tissue engineering due to its biodegradability and biocompatibility. To meet the massive demand for this monomer, an efficient whole-cell biocatalytic approach was constructed boost ε-caprolactone production using cyclohexanol as substrate. Combining alcohol dehydrogenase (ADH) with cyclohexanone monooxyg...

The paper explored the catalytic activity of a cell debris self-immobilized thermophilic lipase for polyester synthesis, using the ring-opening polymerization of ε-caprolactone as model. Effects of biocatalyst concentration, temperature, and reaction medium on monomer conversion and product molecular weight were systematically evaluated. The biocatalyst displayed high catalytic activity at high...

Valorization of the abundant renewable lignocellulose through an efficient chemoenzymatic strategy to produce furan-based platform compounds has raised great interest in recent years. In this work, a newly prepared sulfonated tin-loaded rice husk-based heterogeneous chemocatalyst UST-Sn-RH was utilized transform corncob (75.0 g/L) into furfural (72.1 mM) at 170 °C for 30 min aqueous system. To ...

Photosynthesis-driven whole-cell biocatalysis has great potential to contribute a sustainable bio-economy since phototrophic cells use light as the only energy source. It yet be shown that microorganisms, such cyanobacteria, can combine supply of high heterologous enzyme levels with allocation sufficient reduction equivalents enable efficient light-driven redox biocatalysis. Here, we demonstrat...

We developed a kinetic model that describes a heterogeneous reaction system consisting of a solid substrate suspension for the production of D-amino acid using D-hydantoinase. As a biocatalyst, mass-produced free and whole cell enzymes were used. The heterogeneous reaction system involves dissolution of a solid substrate, enzymatic conversion of the dissolved D-form substrate, spontaneous racem...

α-Ketoisocaproate (KIC) is used widely in the pharmaceutical and nutraceutical industries. In previous studies, we achieved a one-step biosynthesis of KIC from l-leucine, using an Escherichia coli whole-cell biocatalyst expressing an l-amino acid deaminase (l-AAD) from Proteus vulgaris. Herein, we report the fine-tuning of l-AAD gene expression in E. coli BL21 (DE3) at the transcriptional and t...

Background. Production of highly pure enantiomers of vicinal diols is desirable, but difficult to achieve. Enantiomerically pure diols and acyloins are valuable bulk chemicals, promising synthones and potential building blocks for chiral polymers. Enzymatic reduction of ketones is a useful technique for the synthesis of the desired enantiomeric alcohols. Here, we report on the characterization ...

In the present paper, the biodesulfurization of simulated light fuel oil (i.e., dibenzothiophene (DBT) in dodecane) and untreated kerosene with a high total sulfur content has been studied by a native isolated bacterium named Bacillus cereus HN. The influences of various parameters such as the reaction temperature (T), biocatalyst cell density, oil phase fraction (OFP), and initial DBT concentr...

in the present paper, the biodesulfurization of simulated light fuel oil (i.e., dibenzothiophene (dbt) in dodecane) and untreated kerosene with a high total sulfur content has been studied by a native isolated bacterium named bacillus cereus hn. the influences of various parameters such as the reaction temperature (t), biocatalyst cell density, oil phase fraction (ofp), and initial dbt concentr...