dna methylation as epigenetic mark plays a key role in normal differential and developmental processes as well as in dynamic gene regulation at the genomic level. to assess dna methylation pattern in different developmental stages of aeluropus littoralis, methylation sensitive amplified polymorphism (msap) was used. methylation and demethylation status at the ccgg recognition site were tracked ...

Sotalol may induce torsade de pointes through cardiac action potential prolongation, but a proarrhythmic effect secondary to its beta blocking action has not been reported. A 54 year old man presented with symptoms of angina and presyncope, subsequently demonstrated to be associated with coronary spasm. Treatment with sotalol exacerbated his symptoms and resulted in recurrent polymorphic ventri...

We present Creedo—a conceptual framework along with an accompanying software implementation for empirically evaluating knowledge discovery systems. Creedo provides a language for the creation of study designs that test how well different test systems support real users to perform certain data analysis tasks. These designs are scalable and repeatable, i.e., after their creation, a study can be c...

Introduction In recent years, thermal actuated flying height control has been implemented in Hard Disk Drives (HDD) to reduce the effect of flying height variations due to manufacturing tolerances or write current introduced pole tip protrusion (Fig. 1). A stable low flying height is necessary to achieve low bit error rates (BER) [1]. Thermal flying height control is used in a “static manner” i...

Givotia rottleriformis Griff. is an economically important dioecious tree species known for the softwood used in making toys. Knowledge of genetic variation in a dioecious tree species is important for devising strategies for its successful management and conservation. Studies were conducted to examine the genetic variation in Givotia plants using RAPD (random amplified polymorphic DNA) analysi...

The degree to which evolutionary trajectories and outcomes are repeatable across independent populations depends on the relative contribution of selection, chance and history. Population size has been shown theoretically and empirically to affect the amount of variation that arises among independent populations adapting to the same environment. Here, we measure the contribution of selection, ch...

to investigate the genetic diversity of 21 rapeseeds (brassica napus l.), 20 semi-random isj and ssr primers were used. primers produced a total number of 158 (81.5%) polymorphic bands (51 ssr and 107 isj bands). average numbers of polymorphic bands for isj, ssr and combined of them were 10.7, 5.1 and 7.9, respectively. pic values ranged from 0.0150 for specific primer o110d08 to 0.3420 for sem...

DNA amplification fingerprinting (DAF) (1) gives about 40–60 bands per polyacrylamide gel lane, which makes the polymerase chain reaction (PCR) pattern rather complex for band isolation from a gel. The presence of minor amplified bands having only a few base pairs difference from major bands interferes with the product cloning because of co-isolation (3). This fact becomes obvious if a multiply...

The complex flagellar filaments of the LIO8 serogroup member Campylobacter coli VC167 are composed of two highly related subunit proteins encoded by the flaA and flaB genes which share 92% identity. Using oligonucleotide primers based on the known DNA sequence of both the flaA and flaB genes from C. coli VC167 in the polymerase chain reaction, we have shown conservation of both fla genes among ...