A group of five tests utilizing wheat germ and soybean lectins and chromogenic substrates (orthonitrophenyl-beta-D-galactopyranoside, gamma-glutamyl-beta-naphthylamide, and prolyl-beta-naphthylamide derivatives) was used as a rapid (30-min) method for the identification of Neisseria gonorrhoeae. The rapid method agreed with Minitek test results for all 126 N. gonorrhoeae isolates and all 39 non...

The combined application of thin-section and critical-point-drying "fracture-label" is used to determine the pattern of distribution and partition of wheat-germ agglutinin and concanavalin A binding sites on the membrane faces of freeze-fractured exocrine and endocrine rat pancreatic cells. Whereas the exoplasmic face of plasma membrane is preferentially labeled by both lectins, the endoplasmic...

modified and unnatural amino acids into protein are possible.t) However, the relatively small amount of product due to early stopping of the translational reaction has been the primary bottleneck for its application. To overcome this problcm, Spirin and co-workers developed a continuous-fiow cell-free translation system, called the CFCF system, in 1988.2) In this system, the protein synthesis i...

in this study, the growth of two strains pseudomonas fluorescens vupf5 and p. fluorescens vupf5-1 (mutant bacterium similar to mutants of regulatory gacs/gaca system) were evaluated in the organic media. this spontaneous mutation blocked biosynthesis of the antimicrobial compounds hydrogen cyanide, phenazine and exoprotease by biocontrol strain pseudomonas fluorescens vupf5. by using organic me...

Introgressive hybridization has played a crucial role in crop breeding. Powdery mildew-resistant gene 21 (Pm21), located on the short arm of chromosome 6V of Haynaldia villosa (H. villosa), is typically a representative element which is transferred to hexaploid wheat through introgressive hybridization. Determination of sequence evidence for H. villosa DNA introgression would help trace Pm21 in...