Abstract Background Japanese encephalitis virus is a mosquito-borne neurotropic flavivirus that causes acute viral in humans. Pigs are crucial amplifier host of JEV. Recently, increasing evidence has shown long non-coding RNAs (lncRNAs) play important roles infection. Methods JEV proliferation was evaluated after overexpression or knockdown lncRNA-SUSAJ1 using western blotting and reverse-trans...

PURPOSE To elucidate the effect of ouabain on the regulation of proliferation and apoptosis of HUVECs and involvement of different Na(+)-K(+)-ATPase α-subunits and NF-κB. METHODS HUVECs were isolated by collagenase perfusion, and MTT assays and cell cycle analysis were performed to study proliferation. NF-κB expression and function were examined by immunohistochemical staining and western blo...

Trauma-induced suppression of cellular immune function contributes to sepsis, multiple organ dysfunction syndrome (MODS) and mortality. Macrophage migration inhibitory factor (MIF) has been revealed to be central to several immune responses. However, the role of MIF in trauma-like conditions is unknown. Therefore, the present study evaluated MIF in macrophages and polymorphonuclear neutrophils ...

Blotting is a common technique widely used for molecular analysis in life sciences. The Western blot, in particular, is a process of transferring protein samples from a polyacrylamide gel to a blotting membrane and detecting the levels of specific proteins through reactions with primary and secondary antibodies. The state-of-the-art of Western blotting usually generates one blotting membrane pe...

The kidney is considered the most important organ of excretion eliminating the body from metabolic waste products. The nephron is the functional unit of the kidney, composed of a cluster of capillaries named glomerulus, and a tubular system. The blood is filtered in the glomerular capillaries across the glomerular filtration barrier. The barrier is an intricate biological structure composed of ...

background: the effects of extremely low frequency electromagnetic fields (elf-emf) on toxoplasma gondii have not been explained yet. the aim of this study was to assess the possible effects of elf-emf on growth, survival time and viability of toxoplasma gondii . in addition, the life span of toxoplasma infected animals was investigated. methods: sixty adult male balb/c mice were used for in vi...

background: aggressive embryo and receptive endometrium are necessary for successful implantation. on this time endometrium transformates to receptive state, which permits embryonic implantation. studies about embryonic implantation and endometrial receptivity are always a hot spot in the field of reproductive medicine. objective: to investigate the expression pattern of meis1 during peri-impla...