Background Recent researches reveal that manual puncturing of the trophectoderm of blastocyst before vitrification, increase the quality of embryo. However, in any of these studies, the importance of blastocoelic fluid and its impact on the formation of three cell lines is not mentioned. Therefore, in the present study, the effect of blastocoelic fluid reduction before vitrification on survival...

Background In vitro culturing and retransplantion of vitrified- warmed ovarian tissue are two ways to restore fertility after radiation or chemotherapy.This study aimed to evaluate the incidence of apoptosis in vitrified human ovarian tissue after warming, long term culturing and xenotransplantation by morphological analyses and apoptosis genes expression evaluation. MaterialsAndMethods We obta...

The present study has been undertaken to assess the post thaw recovery of buffalo oocytes vitrified at different stages of in vitro maturation (IVM) by propylene glycol and trehalose supplemented with bovine serum albumin (BSA). Cumulus oocyte complexes (COCs) obtained from slaughterhouse ovaries were randomly divided into 6 different groups: control (nonvitrified oocytes were matured for 24 h ...

OBJECTIVE To evaluate the integrity of genomic imprinting in oocytes vitrified at the germinal vesicle (GV) stage and in vitro matured (IVM) after thawing. DESIGN Clinical research and application. SETTING University-based fertility center. PATIENT(S) Immature oocytes were donated for research by patients who were included in an intracytoplasmic sperm injection program. INTERVENTION(S) ...

The effect of modified vitrification was assessed on cellular development capability in mouse embryos cultured in vitro. In this study, 466 embryos (from zygote to morula stages) were vitrified then thawed embryos have been incubated for in vitro farther development up to blastocyst stage. Also, vitrification and thawing procedures were the same for all experimental groups. Mouse different embr...

OBJECTIVES Vitrification is increasingly used in assisted reproductive technology (ART) laboratories worldwide. In this study the effect of vitrification on the expression and modifications of H3 histones of Igf2 and Oct4 was investigated in blastocysts cultured from vitrified and non-vitrified two-cell embryos. MATERIALS AND METHODS In this experimental study, two-cell embryos were cultured ...

BACKGROUND The selection of blastocyst warmed for transfer is based on pre-freeze morphology in vitrified-warmed single blastocyst transfer cycles. But, it is controversial which parameter of blastocyst morphology most closely related to the clinical outcomes. OBJECTIVE To estimate the effect of blastocoele expansion, trophectoderm (TE) morphology grade, and inner cell mass (ICM) morphology g...

The purpose of this study was to find an efficient cryoprotectant and a suitable procedure for vitrification of mouse morulae. The method found with mouse morulae was then applied to the cryopreservation of biopsied goat embryos. Mouse morulae were exposed, in 1or 2-step, to the ethylene glycol (EG)-based solutions EFS30 or EDFS30, which contained 30% EG and 15% EG plus 15% dimethylsulphoxide (...

In nano-confinements, aqueous solutions can be found to remain in a liquid state at subfreezing temperatures. The finding provides a means of entering into previously inaccessible temperature regions for studying the dynamics and structure of bulk liquid. Here we show that studying biomolecular structures in nano-confinements improves the accuracy of cryostructures and provides better insight i...

Background: Oocyte cryopreservation is one of the most important topics in the field of assisted reproductive technology to preserve women fertility, but relationship between cryopreservation and apoptosis is still a matter of debate. The present study was aimed to investigate the effects of vitrification on apoptosis in mouse oocytes by Cryotop method. Method: A total of 200 germinal vesicle (...