Background: Although testicular tissue cryopreservation could be advantageous for fertility preservation in cancerous children, the possibility of apoptosis incidence should not be ignored. Our aim was evaluation of the apoptosis incidence in mouse testicular tissue after vitrification and short-term culture. Materials and Methods: Testes of 7days old NMRI male mice were isolated, divided and d...

Background: Embryo vitrification is a key instrument in assisted reproductive technologies (ARTs). However, thereis increasing concern that vitrification adversely affects embryo development. This study intends to assess the effectof vitrification on developmental competence, in addition to expressions of long non-coding RNA (lncRNA) gene traplocus 2 (Gtl2) and its reciprocal ...

the capability to preserve human oocytes, blastocysts, ovarian tissue and spermatozoa is an important tool in human assisted reproductive techniques. this preservation allows patients undergoing chemotherapy or radiotherapy to preserve their fertility, and helps to attain all benefits from the costly ovarian superovulation therapies prior to art. the primary goal in establishing an appropriate ...

OBJECTIVE To evaluate the safety and risk of cryopreservation in female fertility preservation. DATA SOURCES The data analyzed in this review were the English articles from 1980 to 2013 from journal databases, primarily PubMed and Google scholar. The criteria used in the literature search show as following: (1) human; embryo; cryopreservation/freezing/vitrification, (2) human; oocyte/immature...

OBJECTIVE To investigate the effect of vitrification and beta-mercaptoethanol (beta-ME) on reactive oxygen species (ROS) activity and in vitro development of oocytes vitrified before or after in vitro fertilization (IVF). DESIGN Randomized prospective study. SETTING University-based assisted reproductive technology laboratory. ANIMALS(S): Abattoir-derived porcine ovaries. INTERVENTIONS(S)...

PURPOSE To investigate the effect of antifreeze protein (AFP) supplementation on ovarian vitrification and transplantation. MATERIALS AND METHODS In this experimental study, we researched a total of 182 ovaries from 4-week-old ICR mice. The equilibration solution included 20% ethylene glycol (EG), and the vitrification solution included 40% EG, 18% Ficoll, and 0.3 M sucrose. Intact ovaries we...

Modifying cryopreservation protocols may be seen as a way to simplify cryobanking procedure and increase satisfying outcomes. The aim of the study was to evaluate the influence of slow cooling protocol and vitrification on IVF outcomes using embryos preserved in the 5th or 6th day after oocyte retrieval. The study compared 2 groups of human embryos underwent slow cooling protocol (n=189) and vi...

In this work we analyze the effects of vitrification on the ultrastructure and developmental ability of bovine oocytes matured in the presence of 9-cis-retinoic acid (RA). Bovine cumulus oocyte complexes (COCs) were matured in a basic medium containing 10% fetal calf serum (FCS), 9-cis-RA or polyvinyl alcohol (PVA). Mature oocytes were vitrified using the Open Pulled Straw method with minor mod...

Human induced pluripotent stem (hiPS) cells have great potential for regenerative medicine and drug discovery. It is essential to establish highly efficient and reliable methods for hiPS cell cryopreservation. We examined cryopreservation of hiPS cells by the vitrification method using a dimethyl sulfoxide Me2SO-free and serum-free medium, VS2E, that uses Euro-Collins solution as a base with 40...

Cryopreservation has been recognized as a practical and efficient tool for long-term storage of vegetatively propagated plants. This study was conducted to investigate the effects of sucrose concentration, hardening temperature and different cryopreservation methods on the survival rate of potato shoot tips after cryopreservation. Excised shoot tips of in vitro plantlets of potato cultivars, At...