A fluorogenic-probe hydrolysis (TaqMan)-reverse transcriptase (RT) PCR for classical swine fever virus (CSFV) was evaluated for diagnostic sensitivity and specificity by using clinical samples obtained from the Dominican Republic, where the disease is enzootic. The sensitivity of this test, using nasal swab samples taken from both symptomatic and asymptomatic animals, exceeded the diagnostic se...

This study was directed at the evaluation of the prevalence of bovine viral diarrhea virus (BVDV) infection in bulls in artificial insemination centers. Both serological and virological examinations were performed. Blood samples were tested in micro-seroneutralization test for BVDV antibodies. Virus isolation was performed in cell culture and BVDV antigen was detected in an indirect immunofluor...

After the discovery of 'movement proteins' as a peculiarity of plant viruses and with the help of novel methods for the detection and isolation of interacting host proteins new insights have been obtained to understand the mechanisms of virus movement in plant tissues. Rapid progress in studying the molecular mechanisms of systemic spread of plant infecting viruses revealed an interrelation bet...

We describe the isolation of a paramyxovirus from three species of flying fox in Queensland. The species are Pteropus alecto (black flying fox), Pteropus scapulatus (little red flying fox) and Pteropus poliocephalus (grey headed flying fox). The virus appears to be identical to the equine morbillivirus which was associated with acute equine respiratory syndrome and two human deaths in two Queen...

Influenza virus is rich in variation and mutations. It would be very convenient for virus detection and isolation to histochemically detect viral infection regardless of variation and mutations. Here, we established a histochemical imaging assay for influenza virus sialidase activity in living cells by using a new fluorescent sialidase substrate, 2-(benzothiazol-2-yl)-4-bromophenyl 5-acetamido-...

Sixteen children with asthma were studied for one year and viral isolation attempted during all episodes of wheezing. In 91 episodes investigated, 13 viruses were isolated (isolation rate 14%); whereas only one virus was isolated from 120 specimens taken when the children were symptom free. Rhinovirus was the commonest isolate and most were obtained during August, September, October. Episodes o...

The presence of complement-fixing antibody to an early herpes simplex virus type 2 (HSV-2) antigen (the AG-4 antigen) was correlated with HSV-2 infection in the sera of patients with genital herpes. Eighty-eight per cent of sera taken two weeks after clinical diagnosis of a primary or recurrent herpes infection in patients, confirmed to have HSV-2 by virus isolation and typing, contained the an...

Myxovirus parainfluenza-3 (SF-4 bovine strain) was first isolated in tissue cultures of embryonic bovine kidney (EBK) cells (Reisinger, Heddleston, and Manthei, J. Am. Vet. Med. Assoc. 135:147, 1959). This agent is antigenically and biologically related to the human HA-1 strain isolated previously by Chanock et al. in monkey kidney cells (Abinanti and Huebner, Virology 8:391, 1959; Abinanti et ...

Whole genome amplification and sequencing of single microbial cells has significantly influenced genomics and microbial ecology by facilitating direct recovery of reference genome data. However, viral genomics continues to suffer due to difficulties related to the isolation and characterization of uncultivated viruses. We report here on a new approach called 'Single Virus Genomics', which enabl...

A 2-year field study was conducted in southern Saratoga County, New York, to determine which species of the Aedes communis group mosquitoes were potential vectors of Jamestown Canyon (JC) virus. A total of 23,890 mosquitoes (890 pools) were processed for virus isolation in 1988-89, yielding 17 JC virus isolates from Ae. provocans and one isolate each from Ae. communis, Ae. intrudens and Ae. pun...