IAS is a nonpro f i t organization founded in 1988, with more than 10,000 members representing more than 130 countries. This article addresses three main concepts discussed at the meeting that appear to play a role in the way clini-cians and laboratorians are currently approaching HIV patient management. Viral load blips are temporary spikes in viral load above 50 copies/mL that go back to unde...

BACKGROUND Treatment-naïve patients newly diagnosed with HIV occasionally present with low viral RNA of ≤1'000 copies/ml, raising concerns about viral load underestimation. Because falsely low or undetectable viral loads might lead to inadvertent virus transmission or treatment delays, confirmation of such cases by a sequence-independent viral load test is recommended in Switzerland. METHODS ...

background previous studies using cell culture systems for the replication of hepatitis c virus have opened new research dimensions, and paved the ways for further and detailed studies of the virus in vitro. objectives the purpose of the present study was to cultivate hepatitis c virus in a cell culture system and evaluate viral amplification. materials and methods in order to propagate hepatit...

We examined whether higher human papillomavirus type 16 (HPV16) viral load predicted risk of cervical intraepithelial neoplasia 3 (CIN3) or cancer (together termed zCIN3) within a cohort of 20,810 women followed for 10 years with cytologic screening. Semiquantitative viral load for HPV16 was measured on baseline cervicovaginal specimens using a type-specific hybridization probe test with signal...

We design a "simple" and "low cost" model technology for monitoring HIV viral load in resource-poor settings: SMARThivVLmos. Cost and complexity are the major challenges to the developing world, in monitoring HIV patients viral load. We have previously demonstrated in our SMARThivPack model that cost and complexity of laboratory monitoring of HIV patients, may be reduced not only at a first tec...

background due to a recent alarming increase in the number of hiv-hcv co-infected patients in romania. objectives a cross sectional study was conducted to assess the baseline predictors of liver disease evolution. patients and methods 83 hiv-hcv co-infected patients, untreated for hcv infection, were evaluated for viral replication, liver fibrosis (estimated by a noninvasive marker - fib4), and...

9 We assess the concordance between low level HCV values obtained using the VERSANT HCV RNA 10 1.0 Assay (kPCR) and COBAS AmpliPrep/COBAS TaqMan HCV Quantitative Test v2.0. 11 The correlation between the values obtained by the two RT-PCR assays for samples with quantifiable 12 HCV RNA levels revealed that viral load measured by kPCR significantly correlated with that of the 13 CAP/CTM (R=0.644,...

Risks for sexually transmitted HIV may be related to concentrations of virus detected in semen and previous research shows a small to moderate association between viral load in blood and semen. This study examined the association between viral load in semen and plasma in a community sample of HIV-infected men and is the first study to examine semen viral load in relation to sexual transmission ...

The Abbott RealTime HBV assay targets the N-terminal region of the S gene. Here we analyzed the sequence variability of the assay target region from >2,100 clinical specimens. Thermodynamic modeling of the percentage of bound primer/probe at the assay annealing temperature was performed to assess the potential effect of sequence variability.

BACKGROUND Multiple strains infection of human cytomegalovirus (HCMV) was found to be correlated with increased viral load in immunodeficient patients. However, the pathogenic mechanism underlying this correlation remains unclear. To evaluate genetic polymorphisms of HCMV glycoprotein and their potential role in its viral load, HCMV glycoprotein B, N, and O (gB, gN and gO) genotypes was studied...