We have recently found a novel fatty acid, 11-keto-9(E),12(E)-octadecadienoic acid (KOD), that enhances fibrinolytic activity of endothelial cells. The mechanism of action of KOD has been investigated. KOD increased 2-fold the plasmin activity of bovine aortic endothelial cells at 250 microM. The stimulation was dependent on plasminogen and was inhibited by anti-urokinase, whereas KOD did not e...

The high molecular weight form of the plasminogen activator urokinase (54kD) binds to specific receptor sites on the cell membrane of breast carcinomas by its inactive 'A' chain. The binding is of high affinity (range of dissociation constants: 5.6 x 10-II to 4 x 10 -10 mol and there were between 20 to 250fmol of binding sites per milligram of membrane protein) and equilibrium is reached in 60m...

Whereas crude bovine thrombin activated single-chain urokinase-type plasminogen activator (scu-PA), otherwise called pro-urokinase (pro-UK). purified human thrombin converted pro-UK (scu-PA) to a two-chain form that had no amidolytic activity. The two chains (M 33.000 and 22.000) were disulfide linked and resistant to subsequent activation by plasmin. By contrast. thrombin did not mactivate tis...

The aim of the present study is to elucidate the enzymological and chemical properties of the plasminogen activator in bile, bilokinase. A bilokinase preparation was obtained from 94.2 liters of bovine gall bladder bile through seven purification steps, two of which employed a precipitation method in which ammonium sulfate and acetone were used sequentially. Twenty mg of bilokinase preparation ...

In an attempt to elucidate the mechanism of fibrinolytic enhancement by orally administered urokinase, studies on the intestinal transport of urokinase were carried out, using 125I-labeled human high mol wt urokinase, administered intraduodenally in the experimental dog model with a saphenous vein thrombus. Using the plasma sample obtained from blood 45 minutes after intraduodenal administratio...

Whereas crude bovine thrombin activated single-chain urokinase-type plasminogen activator (scu-PA), otherwise called pro-urokinase (pro-UK). purified human thrombin converted pro-UK (scu-PA) to a two-chain form that had no amidolytic activity. The two chains (M 33.000 and 22.000) were disulfide linked and resistant to subsequent activation by plasmin. By contrast. thrombin did not mactivate tis...

Human and animal sera contain a globulin, plasminogen, which in the presence of activators is rapidly converted to plasmin, a proteolytic enzyme active at neutral hydrogen ion concentrations. Activators specific for plasminogen include streptokinase and staphylokinase of bacterial origin, urokinase and plasma activator found in body fluids, and fibrinokinase derived from tissues. Trypsin, an ac...

Small-cell lung carcinoma releases progalanin. The released progalanin is activated via a nonclassical processing pathway, being processed into an active form of galanin (1-20) by plasmin in extracellular components. Plasmin is produced from plasminogen activators. To clarify the regulation of progalanin via plasminogen activation by urokinase and tissue-plasminogen activator (t-PA), we investi...

Human and animal sera contain a globulin, plasminogen, which in the presence of activators is rapidly converted to plasmin, a proteolytic enzyme active at neutral hydrogen ion concentrations. Activators specific for plasminogen include streptokinase and staphylokinase of bacterial origin, urokinase and plasma activator found in body fluids, and fibrinokinase derived from tissues. Trypsin, an ac...