BACKGROUND Fatty acid binding proteins (FABPs) are members of the intracellular li- pid binding protein (iLBPs) family and most of them show tissue specific expression. FABP9/PERF15 (Perforatorial15) is the male germ cell-specific fatty acid-binding pro- tein. It was first identified as the major constituent of the murine sperm perforatorium and perinuclear theca. To date, investigations in mic...

In a previous study (8) the livers of rats fed the hepatic carcinogen, 4-dimethylaminoazobenzene, were separated by differential centrifugation into the nuclei, large granules (mitochondria), small granules (microsomes), and a supernatant fluid (particles nonsedimentable at 19,000 X g). Anal yses for protein, nucleic acids, riboflavin, and pro tein-bound aminoazo dye were made on each frac tion...

Several members of the mitochondrial carrier protein family are classified as uncoupling proteins. In contrast to the uncoupling protein specific to brown adipose tissue (UCP1), the physiological role of skeletal muscle uncoupling proteins (UCP2 and UCP3) in weight regulation seems more closely associated with the regulation of lipids as fuel substrate than as mediators of adaptive thermogenesis.

BACKGROUND Patients with heart failure show a very high incidence of arrhythmias and sudden death that is often preceded by ischemia; however, data on electrophysiological changes during ischemia in failing myocardium are sparse. We studied electrical uncoupling during ischemia in normal and failing myocardium. METHODS AND RESULTS Tissue resistance, intracellular Ca2+ concentration (Indo-1 fl...

Mitochondria have a fundamental role in the transduction of energy from food into ATP. The coupling between food oxidation and ATP production is never perfect, but may nevertheless be of evolutionary significance. The 'uncoupling to survive' hypothesis suggests that 'mild' mitochondrial uncoupling evolved as a protective mechanism against the excessive production of damaging reactive oxygen spe...

We have used a specific immunoassay for uncoupling protein and [3H]GDP binding to study the acute and chronic responses of brown-adipose-tissue (BAT) mitochondria of warm-acclimated rats to housing at 4 degrees C and cold-acclimated rats to housing at 27 degrees C. These studies have shown the following. (1) In the cold-exposed rat the increase in mitochondrial uncoupling-protein concentration ...

Mild uncoupling of oxidative phosphorylation, caused by a leak of protons back into the matrix, limits mitochondrial production of ROS (reactive oxygen species). This proton leak can be induced by the lipid peroxidation products of ROS, such as HNE (4-hydroxynonenal). HNE activates uncoupling proteins (UCP1, UCP2 and UCP3) and ANT (adenine nucleotide translocase), thereby providing a negative f...

Chemical looping is an innovative technique that relies, to a large extent, on the possibility of finding new oxygen carriers. Until now, these materials have primarily been identified via experimental techniques and therefrom derived insights. However, this both costly time-consuming. To speed-up process, we applied computational screening approach based energetic data retrieved from Open Quan...

Introduction Our laboratory has previously demonstrated that adoptive cell transfer (ACT) of melanoma-specific memory T cells (TM) results in a more potent local and systemic T cell response than ACT with melanoma-specific effector T cells (TE). However, we have also seen that TM are not more cytotoxic than TEin vitro. We hypothesized that a combination of TE+M ACT would have an additive effect...

Animal viral diseases in Latin American countries are studied in the light of the growing economic dependence between industrialized countries and countries producing raw materials, including animal pro­ tein. Viral diseases contribute to create the status of underdevelopment but may also be consequences of this status. The history of the livestock industry in Latin America, where viral disease...