Regenerative mechanisms after surgical injury have been studied in many organs but not in the kidney. Studying surgical injury may provide new insights into mechanisms of kidney regeneration. In rodent models, extrarenal tissues adhere to surgical kidney wound and interfere with healing. We hypothesized that this can be prevented by wrapping injured kidney in a plastic pouch. Adult rats tolerat...

Erythropoietin (EPO) is upregulated by hypoxia and causes proliferation and differentiation of erythroid progenitors in the bone marrow through inhibition of apoptosis. EPO receptors are expressed in many tissues, including the kidney. Here it is shown that a single systemic administration of EPO either preischemia or just before reperfusion prevents ischemia-reperfusion injury in the rat kidne...

A NEMIA OF VARIOUS degrees of severity occurs following lead poisoning. This anemia is usually hypochromic with an increased reticulocyte count and increased red cell hemolysis. These changes are attributed to an inhibition of several enzymes involved in heme synthesis, an interference with the transport ofiron, as well as changes in the erythrocyte membrane.1 A defect in erythroid differentiat...

Alginate hydrogels are a commonly used substrate for in vitro 3D cell culture. These naturally derived biomaterials highly tunable, biocompatible, and can be designed to mimic the elastic modulus of adult brain at 1% w/v solution. Recent studies show that molecular weight alginate affect viability differentiation. The relationship between weight, viscosity ratio G:M monomers is complex, balance...

Urinary indices are classically believed to allow differentiation of transient (or pre-renal) acute kidney injury (AKI) from persistent (or acute tubular necrosis) AKI. However, the data validating urinalysis in critically ill patients are weak. In the previous issue of Critical Care, Pons and colleagues demonstrate in a multicenter observational study that sodium and urea excretion fractions a...

Studies using cellular therapies, scaffolds, and tubular structured implants have been carried out with the goal to restore functional recovery after spinal cord injury (SCI). None of these therapeutic strategies, by themselves, have been shown to be sufficient to achieve complete restoration of function. To reverse the devastating effects of SCI, an interdisciplinary approach that combines mat...

Early in pancreatic development, epithelial cells of pancreatic buds function as primary multipotent progenitor cells (1°MPC) that specify all three pancreatic cell lineages, i.e., endocrine, acinar and duct. Bipotent "Trunk" progenitors derived from 1°MPC are implicated in directly regulating the specification of endocrine progenitors. It is unclear if this specification process is initiated i...

Background & Objectives: Tubular adenomas are rare benign epithelial tumours of breast affecting predominantly females of child bearing age group. Till now, very small number of cases have been reported in the literature. Present study was carried out to evaluate the clinico-pathological features of tubular adenoma cases diagnosed during three years study period along with discussion of possibl...

It was the aim of this study to characterize development gonads and genital ducts in equine fetus around time sexual differentiation. This included identification localization primordial germ cell population. Equine fetuses between 45 60 days gestation were evaluated using a combination micro-computed tomography scanning, immunohistochemistry, multiplex immunofluorescence. Fetal increased size ...

Since the first publication on generating kidney-like cell aggregates from pluripotent stem cells, various modifications have been made to develop more complex and detailed kidney structures. In contrast earlier models that featured nephron-like structures, these advances improved differentiation efficiency similarity human kidney. Presently, organoids contain not only nephrons ureteric buds bu...