Fibers composed of type I collagen triple helices form the organic scaffold of bone and many other tissues, yet the energetically preferred conformation of type I collagen at body temperature is a random coil. In fibers, the triple helix is stabilized by neighbors, but how does it fold? The observations reported here reveal surprising features that may represent a new paradigm for folding of ma...

This article explores the role of universities and their engagement in selected Asian African cities; and, ways which they contribute to developing sustainable cities context major social, cultural, environmental economic challenges facing global south. Drawing on multiple approaches gathering data six case-study cities, SUEUAA (Strengthening Urban Engagement Universities Africa Asia) seeks str...

The hereditary bone disorder osteogenesis imperfecta is often caused by missense mutations in type I collagen that change one Gly residue to a larger residue and that break the typical (Gly-Xaa-Yaa)(n) sequence pattern. Site-directed mutagenesis in a recombinant bacterial collagen system was used to explore the effects of the Gly mutation position and of the identity of the residue replacing Gl...

The folding and assembly of procollagen occurs within the cell through a series of discrete steps leading to the formation of a stable trimer consisting of three distinct domains: the N-propeptide, the C-propeptide and the collagen triple helix flanked at either end by short telopeptides. We have established a semi-permeabilized cell system which allows us to study the initial stages in the fol...

Sequence-specific duplex DNA recognition by oligonucleotide-directed triple helix formation is a possible approach to in vivo gene inhibition. However, triple helix formation involving guanine-rich oligonucleotides is inhibited by physiological ions, particularly K+, most likely due to oligonucleotide aggregation via guanine quartets. Three oligodeoxynucleotide (ODN) derivatives were tested for...

Loet LEYDESDORFF is Professor at the Amsterdam School of Communications Research (ASCoR) of the University of Amsterdam, Visiting Professor of the Institute of Scientific and Technical Information of China (ISTIC) in Beijing, and Honorary Fellow of the Science and Technology Policy Research Unit (SPRU) of the University of Sussex. With Henry Etzkowitz, he initiated a series of conferences, work...

The Triple Helix paradigm – a construct addressing the research-based links between government, universities, and firms – is lacking in formal tests. This study attempts to alleviate this deficiency by conducting an empirical analysis of the effects of relationship-based forms of capital, which has been identified by theorists as an attribute of Triple Helix-based R&D. OLS results utilising a u...

Analysis of business-academia (B-A) collaborations typically relies on a single method, addressing one or two major research questions. In contrast, this article tackles both research and development (R&D) and innovation collaborations among businesses and academia relying on information using multiple methods and multiple sources of information to offer insights on dynamics and qualitative fea...

In this study, by using X-ray powder diffraction profiles as blueprints, we successfully mapped the most probable molecular-level structural arrangement of the PMMA super-helix stereocomplexes through molecular dynamic simulations. Molecular-level resolution of the PMMA triple-helix supramolecule was not previously achievable by experimental methods. After constructing molecular models of stere...