A series of experiments were carried out in order to optimize a protocol for the direct organogenesis of Chilean red clover germplasm. A range of cultivars were used to analyze the effect of explant source (crown or stem meristems of vegetative plants), culture media and plant growth regulators. Our findings showed that stem meristems were easier to obtain, presented lower levels of contaminati...

The oestrogenic activity of certain cultivars of subterranean clover (Trifolium subterraneum, L.) first became known through the observation of serious infertility in ewes in the early 1940's in Western Australia (Bennetts, Underwood and Shier-1946). The manifestations in commercial flocks of the syndrome "clover disease" have been dealt with in the preceding paper (Lightfoot 1974). The deleter...

BACKGROUND AND AIMS Using two parental clones of outcrossing Trifolium ambiguum as a potential model system, we examined how during seed development the maternal parent, number of seeds per pod, seed position within the pod, and pod position within the inflorescence influenced individual seed fresh weight, dry weight, water content, germinability, desiccation tolerance, hardseededness, and subs...

Aim of present study is to evaluate the effect of salinity on the growth of Rhizobium symbiosis with various cultivars of Berseem (WARDAN, BB3, JHB 052 and BB2). Two sets of plots were maintained in which one set contained non-inoculated while another set contained inoculated seeds with Rhizobium culture, which were irrigated with saline waters of different concentrations (0, 3, 6, 7.2, 10, 12 ...

Subterranean clover (Trifolium subterraneum L. cv Woogenellup) and soft chess grass (Bromus mollis L. cv Blando) were grown in monocultures with (15)NH(4)Cl added to the soil to study nitrogen movement during regrowth following shoot removal. Four clipping treatments were imposed. Essentially all available (15)N was assimilated from the soil prior to the first shoot harvest. Measurements of tot...

In perennial grasslands R. obtusifolius (broad-leaved dock) is often surrounded by certain plant species. Thus, it is hypothesized for the current study that Rumex can affect their neighbouring plant species by allelopathic interactions. To test this hypothesis, in a series of laboratory and field experiments aqueous extracts of green R. obtusifolius leaves were sprayed on seeds of 14 herbaceou...

In a feeding trial with 24 sheep, we used the alkanes, long-chain alcohols (LCOH) or both of these plant wax markers, to estimate the diet composition of animals offered diets comprising alkane-labelled cottonseed meal (CSM) together with up to four forages. The diets used were: Diet 1 subterranean clover (Trifolium subterraneum); Diet 2 subterranean clover + phalaris (Phalaris aquatica); Diet ...