Seven fungal isolates were identified as pan-global Hypocrea/Trichoderma species, from section Trichoderma, on the basis of their morphology. These species were H. lixii/T. harzianum and H. orientalis/T. longibrachiatum. PCR-based markers with primer M13 (core sequence of phage M13) and internal-transcribed spacer sequences of ribosomal DNA were used to confirm the identity of the two Trichoder...

In order to find an alternative fungal source, 13 different fungi (Aspergillus, Trichoderma, Penicillium, Rhizopus and Fusarium sp.) were cultured in lactase production medium at 30 °C and 150 rpm for 6 days. Experimental results showed that Trichoderma viride ATCC 32098 has maximum lactase specific activity, followed by Trichoderma harzianum 1073 D3. In addition, the studies of stability were ...

Five novel metabolites, trichodenones A-C (1-3), harzialactone A (4) and B (5), have been isolated together with known R-mevalonolactone (6) from the culture broth of a strain of Trichoderma harzianum OUPS-N115 originally separated from the sponge Halichondria okadai. Their structures have been elucidated by spectral evidence. Among them, 1-3 exhibited significant cytotoxicity against cultured ...

Filamentous fungi from the genus Trichoderma have been widely investigated due to their considerable production of important biotechnological enzymes. Previous studies have demonstrated that the T. harzianum strain IOC-3844 has a high degree of cellulolytic activity. After excluding the native signal peptide, the open reading frame of the T. harzianum endoglucanase III enzyme was cloned in...

Media and culturing protocols were identified that supported the formation of submerged conidia and microsclerotia (MS) by Trichoderma harzianum Rifai strain T-22 using liquid culture fermentation. Liquid media with a higher carbon concentration (36 g L(-1)) promoted MS formation at all C:N ratios tested. Hyphae aggregated to form MS after 2 d growth and after 7 d MS were fully melanized. This ...

A simple detached leaf-droplet inoculation bioassay was devised in order to investigate the potential for biological control of Botrytis cinerea by indigenous Trichoderma harzianum isolates. Following preliminary in vitro screening of the antagonistic capacity of four local isolates of Trichoderma harzianum (T1-T4) against B. cinerea, T3 spore suspensions were then tested for their ability to c...

Trichoderma species are widely used for the commercial production of cellulolytic enzymes. In the present investigation, medium components were optimized using a central composite design and response surface methodology to produce endoglucanase (EG) from Trichoderma harzianum KUC1716. From the various medium components tested, cellulose, soy peptone, and thiamine HCl were selected as the optima...

Towards assessing the biodiversity and biogeography of Trichoderma, we have analyzed the occurrence of Trichoderma species in soil and litter from four areas in China: North (Hebei province), South-East (Zhejiang province), West (Himalayan, Tibet) and South-West (Yunnan province). One hundred and thirty five isolates were grouped according to tentative morphological identification. A representa...

biological control of tomato root-knot nematode, meloidogyne javanica, by the isolate bi of trichoderma harzianum was evaluated in greenhouse and laboratory conditions in tomato cv. king stone. effects of fungal spore suspensions in various densities (103 to 108 spore/ml) were studied on different disease intensities (average of gall diameters, average of root weight and average of stem weight)...

Trichoderma harzianum is well known to exhibit induced systemic resistance (ISR) to Curvularia leaf spot. We previously reported that a C6 zinc finger protein (Thc6) is responsible for a major contribution to the ISR to the leaf disease, but the types of effectors and the signals mediated by Thc6 from Trichoderma are unclear. In this work, we demonstrated that two hydrolases, Thph1 and Thph2, f...