The ability of water-insoluble molecules such as triacylglycerols to partition from oil phases into phospholipid interfaces may be crucial to their hydrolysis by lipases in the aqueous environment of plasma and cells. This study uses high resolution and magic angle spinning 13C NMR spectros-copy to measure the solubility of the 8-carbon medium chain triacylglycerol, trioctanoin, in the lamellar...

During a tuberculosis infection and inside lipid-laden foamy macrophages, fatty acids (FAs) and sterols are the major energy and carbon source for Mycobacterium tuberculosis. Mycobacteria can be found both inside a vacuole and the cytosol, but how this impacts their access to lipids is not well appreciated. Lipid droplets (LDs) store FAs in form of triacylglycerols (TAGs) and are energy reservo...

We generated a highly deuterated cholesterol analog (D38-cholesterol) and demonstrated its use for selective vibrational imaging of cholesterol storage in mammalian cells. D38-cholesterol produces detectable signals in stimulated Raman scattering (SRS) imaging, is rapidly taken up by cells, and is efficiently metabolized by acyl-CoA cholesterol acyltransferase to form cholesteryl esters. Using ...

This paper reports experimental data and the thermodynamic modeling of the liquid-liquid equilibrium systems with triacylglycerols, free fatty acids (obtained from olive oil), ethanol and water. The experimental data were fitted using the UNIQUAC model, in which absolute deviation of 1.28 % and root mean square deviation of 2.10 % between experimental and calculated mass fraction of the compone...

Carbon-13 nuc lear m agnetic re sonance (C N MR) spe ctroscopy w as applied t o de termine the fa tty ac id positional composition of triacylglycerols in vegetable oils with oleic acid percentages “higher and lower than 50%” and with linolenic acid percentages higher than 5%. In particular, conventional C NMR spectroscopy which applies the basic one-pulse sequence for signal averaging, was used...

Secretion of triacylglycerol-enriched very-low-density lipoproteins (VLDLs) from the liver is vital for maintaining plasma lipid homeostasis. However, the process of VLDL assembly and lipidation is not well characterized. Here, we observed that liver of Cideb null mice had higher levels of triacylglycerols accompanied by low level of VLDL secretion. Furthermore, VLDL particles secreted from hep...

A description is given of the development by collaborative study of two standardized methods for the determination of polar compounds in oils and fats by adsorption chromatography using silica minicolumns, and for quantification of polymerized triacylglycerols, oxidized triacylglycerols, and diacylglycerols in polar compounds by high-performance size-exclusion chromatography. The first procedur...

The potato lipase, patatin, has long been thought of as essentially inactive towards triacylglycerols. Recently, technology has been developed to isolate potato proteins in native form as food ingredients at industrial scale. Characterisation of native patatin obtained in this way revealed that this enzyme activity towards triacylglycerols has been underestimated. This enables the application o...

A.) Neutral Acylglycerols and Free Fatty Acids by ESI/MS/MS ....................................... 7 A.1.) Structural characterization of triacylglycerols as lithiated adducts by electrospray ionization mass spectrometry using low-energy collisionally activated dissociation on a triple stage quadrupole instrument. ...................................................................................