Gene translocations play an important role in the plasticity and evolution of bacterial genomes. In this study, we investigated the impact on gene regulation of three genome organizational features that can be altered by translocations: (i) chromosome position; (ii) gene orientation; and (iii) the distance between a target gene and its transcription factor gene ('target-TF distance'). Specifica...

An improved method for the purification of human placental transferrin receptor (Tf-R) was developed. Fresh human placenta was homogenized in cold acetone and the acetone powder was prepared. After the acetone powder had been washed with HEPES buffer, the insoluble proteins containing Tf-R were separated by centrifugation and dissolved in Emulgen 109P-containing buffer. Tf-R was collected by af...

In this study, we characterized tissue factor (TF) expression in mouse hepatocytes (HPCs) and evaluated its role in mouse models of HPC transplantation and acetaminophen (APAP) overdose. TF expression was significantly reduced in isolated HPCs and liver homogenates from TF(flox/flox)/albumin-Cre mice (HPC(ΔTF) mice) compared with TF(flox/flox) mice (control mice). Isolated mouse HPCs expressed ...

Cadmium (Cd) contamination harms plant growth and human health. The application of hemin (ferroprotoporphyrin IX) can effectively relieve abiotic stresses in plants. This work investigates the effects on alleviating Cd toxicity enhancing tolerance maize seedlings. In this study, seedlings were cultivated nutrient solutions, with a combination CdCl2 (464 ?mol L?1) (100 L?1). We measured status, ...

Hemorrhagic complications are common among hemodialysis (HD) patients. The mechanisms by which HD perturbs the coagulation cascade are still being defined. This study evaluated the influence of HD serum on cellular expression of tissue factor (TF), a procoagulant membrane-associated protein that is a pivotal regulator of blood coagulation. Serum was collected immediately before dialysis and 15,...

In order to investigate the accumulation and bio-absorption of lead and cadmium in radish and cress, the present study has been conducted in a completely randomized design in three replicates in a hydroponic growing medium. The first factor includes the plant type at two levels (radish and cress), and the second factor is consisted of lead (Pb) (first experiment) at two levels (50 and 100 mg/L)...

Cultured endothelial cells can be induced by tumor necrosis factor/cachectin (TNF) and other cytokines to synthesize the procoagulant cofactor tissue factor (TF). Intact monolayers of TNF-treated endothelial cells showed only minimal TF activity. In contrast, after permeabilization of these monolayers with detergent (saponin, 0.02%), there was approximately 10- to 20-fold increase in TF-mediate...

Mitigating heavy metals (HMs) contamination and ensuring the safe production of crops is paramount importance for sustainable agriculture development. The purpose current field plot study was to select maize varieties with low HMs metalloids in their edible parts but high accumulation other parts. cadmium (Cd), arsenic (As), lead (Pb), chromium (Cr) contents 11 were measured by atomic absorptio...

The objectives of this study were to test the strain Wickerhamomyces anomalus WO2 as a plant growth-promoting yeast (PGPY), evaluate effect its inoculation on growth and physiological performance Suaeda fruticosa subjected salt stress, understand by which mechanism can protect from high salinity. results showed that is halophilic, grows at concentrations up 15%. Salinity caused decreases in chl...

Abstract Discharge of untreated industrial effluents has been associated with soil contamination. Biochemical alterations in Lumbricus terrestris and remediation capacity Azadirachta indica tree from effluent discharge locations Challawa (A B) Kura village (control location C) were assessed. Levels nitrate, phosphate, iron, chloride, hardness at A B significantly ( p < 0.05) higher than thos...