Background: Transgenic plants inhabiting single Bt gene are prone to develop insect resistance and this resistance has been reported in case of some important yield-devastating insect larvae of commercial crops, such as cotton and rice. Therefore, it has become essential to adapt new strategies to overcome the problem of insect resistance and these new strategies should be sophisticated enough ...

Research was conducted to quantify the development of the corn earworm (= bollworm), Helicoverpa zea (Boddie), on two different transgenic cotton cultivars (DP 50B and NuCOTN 33B) that contained different levels of the Cry1Ac endotoxin from the soil bacterium, Bacillus thuringiensis Berliner. Using a field cage, an inverse relationship between the amount of Cry1Ac among cultivars versus the wei...

Transgenic Bt-cotton expresses insecticidal Cry proteins via Cry genes, introduced from the soil bacterium Bacillus thuringiensis (Bt). Data on levels of Cry proteins expressed in transgenic Bt-crops is important for assessing levels of environmental exposure. During investigations into the rhizosphere impacts of Cry proteins from GM cotton we found that Cry1Ac expression throughout the season ...

The adoption of transgenic Bt cotton has, in some cases, led to environmental and economic benefits through reduced insecticide use. However, the distribution of these benefits and associated risks among cotton growers and cotton-growing regions has been uneven due in part to outbreaks of non-target or secondary pests, thereby requiring the continued use of synthetic insecticides. In the southe...

The phytosynthesis of nanoparticles is a green chemistry approach that combines nanotechnology and bioactive compounds of plants. The aim of this study was to evaluate the effect of silver nanoparticles (AgNPs) from Prosopis glandulosa and Pluchea sericea, respectively, on the control of Fusarium solani previously inoculated in the rhizosphere of transgenic insect-resistant cotton plants. The r...

Cholesterol oxidase represents a novel type of insecticidal protein with potent activity against the cotton boll weevil (Anthonomus grandis grandis Boheman). We transformed tobacco (Nicotiana tabacum) plants with the cholesterol oxidase choM gene and expressed cytosolic and chloroplast-targeted versions of the ChoM protein. Transgenic leaf tissues expressing cholesterol oxidase exerted insectic...

low phosphorous (p) availability in soils limits production of soybean [glycine max (l.) merr.] around the world. this study was conducted to determine whether exogenous expression of the rice (oryza sativa l.) phosphates transporter gene ospt2 would increase inorganic phosphates (pi) acquisition and improve yield in transgenic soybean. cotyledonary-node explants of the soybean were inoculated ...

BACKGROUND Programmed cell death plays an important role in mediating plant adaptive responses to the environment such as the invasion of pathogens. Verticillium wilt, caused by the necrotrophic pathogen Verticillium dahliae, is a serious vascular disease responsible for great economic losses to cotton, but the molecular mechanisms of verticillium disease and effective, safe methods of resistan...

Cotton production is hampered by a variety of abiotic stresses that wreak havoc on the growth and development plants, resulting in significant financial losses. According to reports, cotton areas have declined around world as result ongoing stress. Therefore, plant breeding programs are concentrating stress-tolerant varieties. Mitogen-activated protein kinase (MAPK) cascades involved growth, st...

The conserved coat or V2 gene of begomoviruses is responsible for viral movement in the plant cells. RNAi technology was used to silence V2 gene for resistance against these viruses in transgenic plants. The transformation of the RNAi-based gene construct targeting V2 gene of CLCuKoV-Bur, cloned under 35S promoter, was done in two elite cotton varieties MNH-786 and VH-289 using shoot apex cut m...