enzyme electrophoresis was used to measure genetic variation within, and divergence among, three generations of recently bred synthetic alfalfa generations (syn1, syn2, and syn3) originating from a polycross of 12 selected parents and several cultivars. three isozyme loci, exhibiting tetrasomic inheritance in 10-day seedlings, were detected from five enzymatic systems analyzed by polyacryla...

To more fully characterize the internal structure of transgene loci and to gain further understanding of mechanisms of transgene locus formation, we sequenced more than 160 kb of complex transgene loci in two unrelated transgenic oat (Avena sativa L.) lines transformed using microprojectile bombardment. The transgene locus sequences from both lines exhibited extreme scrambling of non-contiguous...

We have analyzed somatic hypermutation of an immunoglobulin (Ig) heavy chain transgene. Hybridomas expressing the transgene were produced from immunized transgenic mice and transgene copies were sequenced to assay for mutation. In two IgM-producing hybridomas, as well as in several IgG-producing hybridomas, mutations were found in the VDJ region of the transgene. In the IgM-producing hybridomas...

A protocol to produce transgenic shoots of Malus X domestica cv Greensleaves was optimized using two gene constructs previously used create parthenocarpic tomato, Ino-IaaM and DefH9-IaaM. The aim obtain sufficient nº for in vitro multiplication, transfer soil, grafting testing parthenocarpy the next years. We investigated effects modifications a previous published protocol: 1) co-transformation...

BLG/7 transgenic mice express an ovine beta-lactoglobulin transgene during lactation. Unusually, transgene expression levels in milk differ between siblings. This variable expression is due to variegated transgene expression in the mammary gland and is reminiscent of position-effect variegation. The BLG/7 line was created and maintained on a mixed CBA x C57BL/6 background. We have investigated ...

background: a highly efficient genetic transformation system is essential for a successful genetic manipulation of the african violet (saintpaulia ionantha wendl.).objectives: developing a particle bombardment-based genetic transformation system for the african violet.materials and methods: a local cultivar of the african violet from guilan province was used for transformation experiments. the ...

identification of genes with invariant levels of gene expression is a prerequisite for validating transcriptomicchanges accompanying development. ideally expression of these genes should be independent of themorphogenetic process or environmental condition.we report here the validation of internal control genei.e.tps (trehalose 6-phosphate-synthase) in cotton (gossypium spp), using taqman syste...

alport syndrome is a progressive hereditary nephritis leading to renal failure. nearly all of the documents declare that alport syndrome is inherited as x-linked dominant trait and reports of autosomal inheritance form is very rare. this paper presents an iranian large alport family with autosomal recessive inheritance. in our patients alport disease was confirmed with electron microscopic stud...