During eukaryotic DNA interstrand cross-link (ICL) repair, cross-links are resolved ("unhooked") by nucleolytic incisions surrounding the lesion. In vertebrates, ICL repair is triggered when replication forks collide with the lesion, leading to FANCI-FANCD2-dependent unhooking and formation of a double-strand break (DSB) intermediate. Using Xenopus egg extracts, we describe here a replication-c...

Defects in the repair of DNA interstrand crosslinks (ICLs) are associated with the genome instability syndrome Fanconi anemia (FA). Here we report that cells with mutations in RFWD3, an E3 ubiquitin ligase that interacts with and ubiquitylates replication protein A (RPA), show profound defects in ICL repair. An amino acid substitution in the WD40 repeats of RFWD3 (I639K) found in a new FA subty...

Idiopathic CD4 lymphocytopenia (ICL) is characterized by a low CD4+ lymphocyte count in the absence of HIV or other underlying etiologies. We report a case of a 57-year old man with ICL and giant cell arteritis (GCA) who developed pulmonary mucormycosis, which, to our knowledge, is the first report of these occurring in a patient with ICL. Abnormally low total lymphocyte or CD4+ cell counts occ...

As a variant of Shokurov’s criterion of toric surface, we give a criterion of two new classes of normal projective surfaces, called pseudo-toric surfaces of defect one and half-toric surfaces. A typical example of pseudo-toric surface of defect one is the blown up of a projective toric surface at a nonsingular point of the boundary divisor. A half-toric surface is the quotient of a projective t...

The purpose of this paper and its sequel [GS11b] is to introduce and develop a theory of toric stacks which encompasses and extends the notions of toric stacks defined in [Laf02, BCS05, FMN10, Iwa09, Sat12, Tyo12], as well as classical toric varieties. In this paper, we define a toric stack as the stack quotient of a toric variety by a subgroup of its torus (we also define a generically stacky ...

Kang, Sok Han, Pieter Vanden Berghe, and Terence K. Smith. Ca2 -activated Cl current in cultured myenteric neurons from murine proximal colon. Am J Physiol Cell Physiol 284: C839–C847, 2003. First published November 27, 2002; 10.1152/ajpcell.00437.2002.—Whole cell patchclamp recordings were made from cultured myenteric neurons taken from murine proximal colon. The micropipette contained Cs to r...

During replication-coupled DNA interstrand crosslink (ICL) repair, the XPF-ERCC1 endonuclease is required for the incisions that release, or "unhook", ICLs, but the mechanism of ICL unhooking remains largely unknown. Incisions are triggered when the nascent leading strand of a replication fork strikes the ICL Here, we report that while purified XPF-ERCC1 incises simple ICL-containing model repl...

The ion-channel laser (ICL) has been proposed as an alternative to the free-electron laser (FEL), replacing the deflection of electrons by the periodic magnetic field of an undulator with the periodic betatron motion in an ion channel. Ion channels can be generated by passing dense energetic electron bunches or intense laser pulses through plasma. The ICL has potential to replace FELs based on ...

Mitomycin C induces both MC-mono-dG and cross-linked dG-adducts in vivo. Interstrand cross-linked (ICL) dG-MC-dG-DNA adducts can prevent strand separation. In Escherichia coli cells, UvrABC repairs ICL lesions that cause DNA bending. The mechanisms and consequences of NER of ICL dG-MC-dG lesions that do not induce DNA bending remain unclear. Using DNA fragments containing a MC-mono-dG or an ICL...