We have used time-lapse digital imaging microscopy to examine cytoplasmic astral microtubules (Mts) and spindle dynamics during the mating pathway in budding yeast Saccharomyces cerevisiae. Mating begins when two cells of opposite mating type come into proximity. The cells arrest in the G1 phase of the cell cycle and grow a projection towards one another forming a shmoo projection. Imaging of m...

tip leakage loss introduces major part of losses of the rotor in axial gas turbines. therefore, the rotor blade tip has a considerable effect on rotor efficiency. to understand the flow physics of the rotor tip leakage, we solve the flow field for different tip platforms (passive flow control) and by considering coolant tip injection (active flow control). various blade tip configurations such ...

Tip leakage loss introduces major part of losses of the rotor in axial gas turbines. Therefore, the rotor blade tip has a considerable effect on rotor efficiency. To understand the flow physics of the rotor tip leakage, we solve the flow field for different tip platforms (passive flow control) and by considering coolant tip injection (active flow control). Various blade tip configurations s...

BACKGROUND Re-establishing nasal tip projection is one of the main objectives in secondary rhinoplasty. The columellar strut is used as a basic element for support and the septal cartilage graft as the first choice for reconstruction. Nevertheless, the septal cartilage is often missing in this category of patient because of previous surgery, obliging the surgeon to seek a safe, effective, and v...

Cohesive zone failure models are widely used to simulate fatigue crack propagation under cyclic loadings, but the functional form of such phenomenological does not have a strong micromechanical basis. While numerical techniques been developed extract crack-tip cohesive law for monotonic fracture from experimental strain information, elucidating unloading-reloading hysteresis representing accumu...

To identify additional cell fusion genes in Saccharomyces cerevisiae, we performed a high-copy suppressor screen of fus2Delta. Higher dosage of three genes, BEM1, LRG1, and FUS1, partially suppressed the fus2Delta cell fusion defect. BEM1 and FUS1 were high-copy suppressors of many cell-fusion-defective mutations, whereas LRG1 suppressed only fus2Delta and rvs161Delta. Lrg1p contains a Rho-GAP ...

Association of messenger RNAs with large complexes such as processing bodies (PBs) plays a pivotal role in regulating their translation and decay. Little is known about other possible functions of these assemblies. Exposure of haploid yeast cells, carrying mating type "a," to "α pheromone" stimulates polarized growth resulting in a "shmoo" projection; it also induces synthesis of "a pheromone,"...