Thiamine is an essential component of the human diet and thiamine diphosphate-dependent enzymes play an important role in carbohydrate metabolism in all living cells. Although the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe can derive thiamine from biosynthesis, both are also able to take up thiamine from external sources, leading to the down-regulation of the enzymes involved...

We identified a strain carrying a recessive constitutive mutation (thi80-1) with an altered thiamine transport system, thiamine-repressible acid phosphatase, and several enzymes of thiamine synthesis from 2-methyl-4-amino-5-hydroxymethylpyrimidine and 4-methyl-5-beta-hydroxyethylthiazole. The mutant shows markedly reduced activity of thiamine pyrophosphokinase (EC 2.7.6.2) and high resistance t...

background: thiamine (vitb1) is a vitamin with various important physiological functions and postulated therapeutic effects.  its use as an analgesic in neuropathic pain has been undergoing in clinical settings. however, there has been little experimental investigation on this effect. in this study, anti-nociceptive and anti-inflammatory effects of thiamine were investigated in mice. methods: t...

To clarify the effect of thiamine on serotonin-related metabolism in magnesium-deficient rats, in vivo and in vitro experiments were carried out. In thiamine-excess magnesium-deficient and thiamine-adequate magnesium-deficient rats, serotonin oxidation activity in liver and stomach decreased significantly, while a decrease was not observed in thiamine-deficient magnesium-deficient rats. In vitr...

a pot experiment was conducted to evaluate the effect of foliar spray of salicylic acid (0, 50, 100 ppm), ascorbic acid (0, 100, 200 ppm) and thiamine (0, 50, 100 ppm) on vegetative growth, flowering and photosynthetic pigments of marigold (calendula officinalis l.) plants at greenhouse of sb university of kerman, iran. results showed that application of salicylic acid and thiamine increased nu...

In Escherichia coli, thiamine pyrophosphate is synthesized from thiamine monophosphate. Free thiamine is not involved as an intermediate in de novo synthesis of thiamine pyrophosphate.

their keto acid derivatives, leading to metabolic encephalopathy and progressive neurodegeneration in untreated patients [1]. It has an estimated prevalence of 1 in 185,000 worldwide [1, 2] and of 1 in 230,000 in Korea [3]. Disease-causing mutations have been identified in the BCKDHA, BCKDHB, and DBT genes, which encode the E1α, E1β, and E2 subunits of the BCKDH complex, respectively [2]. MSUD ...

The activities of pyruvate and 2-oxoglutarate dehydrogenases were determined by a spectrophotometric method according to Massey, that of transketolase deter mined by Racker's method. The values of enzyme activity as well as the thiamine contents in the preparations were compared with those of the animals whose deficiencies were restored by thiamine administration. The thiamine deprivation resul...