introduction: the purpose of this study was to evaluate the effect of different doses of retinoic acid on resumption of meiosis, in vitro maturation of oocytes and resulting embryo development in mice. materials and methods: germinal vesicle of nmri female mouse (6-8 weeks old) oocytes were collected from ovaries and cultured in maturation medium mem-α contained: 100 mliu/ml rfsh + 7.5 iu/ml hc...

background   objective:   materials and methods   results   conclusion   key words:   . vitrification, viability, in vitro maturation, gvbd, oocyte, mouse : these results suggest that step-wise vitrification of gvbd oocytes as compared to single step vitrification was better in the rate of survival and in vitro maturation of oocytes. : all non-vitrified oocytes were viable after 24 hr; however,...

this study presents the first details on morphological and histological characteristics of gonads, gonadal development stages and reproductive index of the persian loach, oxynoemacheilus persa (heckel, 1847), an iranian endemic species. sampling was done from april 2010 to april 2011 using electrofishing device and a total of 196 individuals were collected. the gonads of specimens were removed,...

background: ovarian tissue transplantation is emerging technologies for fertility preservation. in addition, in vitro maturation (ivm) of oocytes retrieved from ovarian tissues may overcome the fertility defects in certain cases. objective: the aim was to evaluate the best site for ovarian tissue transplantation in mice. also, feasibility of ivm of oocytes retrieved from auto grafted ovarian ti...

The aims of the present study were to assess the effects of cysteamine as an anti-oxidant on the rate of in vitro maturation (IVM) of buffalo oocytes (experiment 1), and their viability and nuclear status following vitrification (experiment 2). Immature oocytes with compact cumulus cells obtained from the ovaries of slaughtered animals were harvested and then cultured in the maturation medium w...

background:   objective: in this study, we supplemented a chemically defined medium with different combinations of selenium, calcium and calcium ionophore concentrations to obtain the best rate of human oocytes maturation, survival, and fertilization.   materials and methods: as an experimental study, three combinations of [selenium (5 μg/ml), calcium (5 μg/ml) and calcium ionophore (1 μg/ml)],...

BACKGROUND AND AIMS Actively growing post-embryonic sporophytes of desert mosses are restricted to the cooler, wetter months. However, most desert mosses have perennial gametophytes. It is hypothesized that these life history patterns are due in part to a reduced thermotolerance for sporophytes relative to gametophytes. METHODS Gametophytes with attached embryonic sporophytes of Microbryum st...

Background: The buffalo (Bubalus bubalis) is the animal with special production characteristics in producing milk and meat has a critical role in rural industry in Iran. The most problem of this specie is its low reproductive output. The aim of the present study was to evaluate the effect of bovine cumulus co-culture on maturation rate of Buffalo oocyte.Materials and Methods: Ovaries were colle...

Background: In vitro culture of ovarian follicles may preserve fertility in women with premature ovarian failure due to cancer .It seems that creation a condition that could maintain cellular communications and supports growth of follicles to produce mature oocytes appear to be essential. Nitric oxide (NO) has been recently shown to act with a dual action in mouse oocyte meiotic maturation depe...

the majority of cancer treatments are invasive. gonadal injuries cause reductions in fertility which results in lack of hope for conception in cancer patients and frustration for their partners. fortunately, current advancements in cryopreservation and transplantation sciences regarding fertility preservation lead to cryostorage of gonads and preservation prior to the onset of chemo- and radiot...