To measure apolipoprotein A-I, we use an RIA kit (Ventrex Laboratories, Inc., Portland, ME) for which serum is recommended, and avoidance of repeated freeze-thaw cycles. With the reported association of alterations in apolipoprotein A-I concentrations in plasma with both heart (1) and liver disease (2), those interested in retrospectively measuring apolipoprotein A-I with this kit have been lim...

The chemical stability of repository compounds is affected by various environmental conditions during long-term storage. Studies were carried out to evaluate the effects of the following potential causes of instability of compounds in DMSO at a 10-mM concentration: water, oxygen, freeze/thaw cycles, and storage container material. A set of compounds was selected for the study based on structura...

The freezing-thawing effect on the meat quality of whole, gutted and fillets of sea bass (Dicentrarchus labrax) were investigated. It was aimed to investigate the changes in the meat quality of whole, gutted and fillets of sea bass in multiple frozen (-18±2ºC) and thawed cycles in refrigerated conditions (4±2°C). The meat quality assessment of the sea bass groups (whole, gutted and fillets) sub...

The technique of using clay-sand mixtures as liners has been widely used in several geotechnical projects where hydraulic barriers or waste-buffer layers are required. During weather fluctuations and below-zero temperature conditions, these can be subjected to successive freeze-thaw cycles. serviceability such needs examined during their lifetime for efficiency function under suspected conditio...

OBJECTIVE To study the effect of freeze-thaw on embryos derived from intracytoplasmic sperm injection (ICSI) using surgically retrieved and ejaculated spermatozoa. DESIGN Retrospective study. SETTING Private IVF center. PATIENT(S) Three hundred eighty-three patients undergoing frozen-thawed ET cycles. INTERVENTION(S) Testicular sperm aspiration (TESA) or percutaneous epididymal sperm as...