The concentration of adenosine 3',5'-monophosphate (cyclic AMP) and the activity of adenylate cyclase were determined for the first time in conjuncation with cyclic 3',5'-nucleotide phosphodiesterase (phosphodiesterase) during the growth cycle of Tetrahymena pyriformis. High levels of cyclic AMP observed during early exponential and late stationary phases were associated with elevated adenylate...

In recent years molecular heterogeneity of proteins has often been observed, particularly for enzymes, where the existence of multiple molecular forms has been extensively domumented ( Wrbblewski, '61). Markert and Mdler ('59) introduced the concept of an isozyme, which they defined as one of the "molecular forms in which proteins may exist with the same enzymatic specificity" within a single o...

The effect of vincristine sulfate (VCR)2 on the incorporation of isotopic precursors into protein, RNA, and DNA of the pro tozoan Tetrahymena pyriformis has been studied. DNA synthesis in logartithmically growing cells of T. pyriformi.s was almost completely inhibited by concentrations of VCR which only moderately depressed RNA and protein synthesis. Under ap propriate conditions this selective...

We exposed division-synchronized Tetrahymena pyriformis to pulse treatments with the structural amino acid analogue p-fluorophenylalanine. We related the timing of the treatments to the delays in onset or completion of division, or both. The results enable us to distinguish between early and late phases in cell division in which the cells bring into action proteins which contribute to initiate ...

Fluorescent acid-fast microscopy (FAM) was used to enumerate intracellular Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum in the ciliated phagocytic protozoan Tetrahymena pyriformis. There was a linear relationship between FAM and colony counts of M. avium cells both from cultures and within protozoa. The Ziehl-Neelsen acid-fast stain could not be used to enum...

Artificial magnetotactic Tetrahymena pyriformis GL (T. pyriformis) cells were created by the internalization of iron oxide nano particles and became controllable with a time-varying external magnetic field. Thus, T. pyriformis can be utilized as a cellular robot to conduct micro-scale tasks such as transportation and manipulation. To complete these tasks, loading inorganic or organic materials ...

In view of the present increased interest in the metabolism of the ciliate, Tetrahymena gdeii, an investigation of the relative activity of the anaerobic dehydrogenases which have a direct role in carbohydrate oxidation was undertaken as the first phase of a general survey of enzymatic activity in this organism. The presence in the ciliate of the dehydrogenases active in the tricarboxylic acid ...

During a parasitological investigation on the fresh water fishes of North West of Iran (West Azerbaijan Province), 266 specimens of 11 fishes were examined. Fishes were collected from seven stations from Aras, Zangbar and Sarysou Rivers between summer 2001 to summer 2002. Nine protozoan species were detected; eight Cilliophora and one Rhizopoda. Cilliophora are: Chilodonella sp < em>., Apiosoma...