Homologous recombination-mediated genome engineering has been broadly applied in prokaryotes with high efficiency and accuracy. However, this method is limited in realizing larger-scale genome editing with numerous genes or large DNA fragments because of the relatively complicated procedure for DNA editing template construction. Here, we describe a CRISPR-Cas9 assisted non-homologous end-joinin...

We report the synthesis of platinum telluride nanoparticles (Pt(3)Te(4) NPs) in the solution phase at room temperature using a template-assisted method. The dendrimeric aggregates formed are composed of several small units of Pt(3)Te(4) NPs of ∼4 nm diameter. Tellurium nanowires (Te NWs) are used as the template and the reducing agent in the growth of NPs which occurs due to the galvanic replac...

Background: Electromyographic (EMG) signals obtained from a contracted muscle contain valuable information on its activity and health status. Much of this information lies in motor unit potentials (MUPs) of its motor units (MUs), collected during the muscle contraction. Hence, accurate estimation of a MUP template for each MU is crucial. Objective: To investigate the possibility of improv...

Abstract In this paper, a series of meso-macroporous silica (MMS) were prepared by phase separation, then mic-meso-macroporous Beta zeolite was steam-assisted crystallization (SAC) method using the as substrate. The factors such amount water, time, aluminum source, template agent during SAC investigated. Various characterization methods BET, XRD, SEM used to investigate physical and chemical pr...

ultrasonic-assisted method was applied for preparation of cu+2-doped zns nanoparticles (mole fractions of cu+2 ions are 0.000, 0.015, 0.030 and 0.060) in water as a template-free and green method at 30 min. the prepared nanoparticles were investigated by powder x-ray diffraction (xrd), scanning electron microscopy (sem), energy dispersive x-ray (edx) and uv–vis diffuse reflectance spectra (drs)...

While the current supramolecular syntheses of [2]rotaxanes are generally efficient, the final product always retains the functional groups required for non-covalent preorganization. A short and high-yielding covalent-template-assisted approach is reported for the synthesis of a [2]rotaxane. A terephthalic acid template core preorganizes the covalently connected ring precursor fragments to induc...

In this paper, the authors present and discuss a model for Cu-Se nano resonant tunneling diodes (RTDs) fabricated by negative template assisted electrodeposition technique and formulate the mathematical equations for it. The model successfully explains the experimental findings.

With an ordered polystyrene (PS) template-assisted electrochemical approach we synthesized three-dimensional ordered macroporous (3DOM) Cu2O/Ni inverse opals as electrodes for supercapacitors. The 3DOM Cu2O/Ni electrodes display superior kinetic performance, and satisfactory rate capability and cycling performance.

Microspheres with controlled nano- and macroporosity are fabricated by template-assisted spray drying. Increasing the porosity of the particle up to 20% improves the rate performance of the particles as shown experimentally and by electrochemical simulations of particle lithiation.