نتایج جستجو برای: targeting cassette
تعداد نتایج: 157564 فیلتر نتایج به سال:
When copyright law developed producing infringing copies was a matter which was made very difficult as a result of the expense and expertise required. Advances in modern technology such as the photocopier, the cassette tape recorder, CD writers and DVD recording machines have made copying a cheap and fast process. Computer technology has produced a new environment which not only facilitates the...
background: among all common techniques in sitedirectedmutagenesis, λ red recombinase system has beenwidely used to knock out chromosomal genes in bacteria. in thismethod, there is always the risk of dna linear digestion byhost's restriction enzymes that leads to the low frequency ofrecombination. objectives:to overcome this, we constructeda recombinant vector to disrupt phop gene in salmonella...
We present an efficient fully automated sodium [18F]fluoride radiosynthesis on a new iMiDEV™ cassette-based microfluidic platform.
Alternative splicing (AS) is a major engine that drives proteome diversity in mammalian genomes and is a widespread cause of human hereditary diseases. More than 95% of genes in the human genome are alternatively spliced, and the most common type of AS is the cassette exon. Recent discoveries have demonstrated that the cassette exon plays an important role in genetic diseases. To discover the f...
KEY POINTS This study explores the molecular mechanisms that regulate the recycling of chromophore required for pigment regeneration in mammalian cones. We report that two chromophore binding proteins, retinol dehydrogenase 8 (RDH8) and photoreceptor-specific ATP-binding cassette transporter (ABCA4) accelerate the dark adaptation of cones, first, directly, by facilitating the processing of chro...
BACKGROUND In order to provide a system fully integrated with qPCR screening, usually used in GMO routine analysis, as well as being able to detect, characterize and identify a broad spectrum of GMOs in food/feed matrices, two bidirectional DNA walking methods targeting p35S or tNOS, the most common transgenic elements found in GM crops, were developed. These newly developed DNA walking methods...
Targeted alteration of the genome lies at the heart of the exploitation of S. pombe as a model system. The rate of analysis is often determined by the efficiency with which a target locus can be manipulated. For most loci this is not a problem, however for some loci, such as fin1+, rates of gene targeting below 5% can limit the scope and scale of manipulations that are feasible within a reasona...
Small genetic elements conferring resistance to antibiotics are widely used for strain construction in bacteria. Here, we describe a gentamicin resistance cassette with properties optimized for efficient gene replacement in Escherichia coli. This cassette offers advantages in versatility and ease of use over previously described versions. The Red recombination system of bacteriophage λ, in the ...
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