This study evaluated the effect of melatonin supplementation of in vitro maturation media on in vitro maturation (IVM) and in vitro fertilization (IVF) rate of buffalo oocytes. Cumulus oocytes complexes (COCs) were aspirated from follicles of 2-8 mm diameter. In experiment I, COCs were matured in IVM medium supplemented with 0 (control), 250, 500, and 1000 μM melatonin for 22-24 hours in CO2 in...

In our previous report, we demonstrated that the tyrosine phosphorylation of sperm proteins (TPSP) of guinea pig was associated with capacitation and hyperactivation (CAHA), and Ca and HCO3 were required for the initiation of CAHA and increasing the TPSP. The aim of this study was to further investigate the mechanism underlying the above events. The results showed that addition of cAMP agonists...

Purpose: investigate the effect of IBMX (activator protein phosphorylation) and prolactin (PRL) on functional state cryopreserved bovine spermatozoa using inhibitory analysis. Materials methods. Frozen-thawed semen samples from 60 black-and-white bulls was used in experiments. For capacitation, cells were incubated Sp-TALP medium supplemented with 6 mg/ml serum albumin various compounds: an ind...

MOTIVATION Correlated events of gains and losses enable inference of co-evolution relations. The reconstruction of the co-evolutionary interactions network in prokaryotic species may elucidate functional associations among genes. RESULTS We developed a novel probabilistic methodology for the detection of co-evolutionary interactions between pairs of genes. Using this method we inferred the co...

As diferentes tarefas de análise morfossintáctica têm muita importância para posteriores níveis do processamento da linguagem natural. Por isso, estes processos devem ser realizados com ferramentas que garantam bons desempenhos em relação à cobertura, precisão e robustez na análise. FreeLing é uma suite com licença GPL desenvolvida pelo Grupo TALP da Universitat Politècnica de Catalunya. Este s...

This work aims to improve an N-gram-based statistical machine translation system between the Catalan and Spanish languages, trained with an aligned Spanish– Catalan parallel corpus consisting of 1.7 million sentences taken from El Periódico M. Farrús (&) M. R. Costa-jussà J. B. Mariño M. Poch A. Hernández C. Henrı́quez J. A. R. Fonollosa TALP Research Center, Department of Signal Theory and Comm...

Glycosaminoglycans (GAG) promote acrosome reactions (AR) in bovine and rabbit spermatozoa in vitro. Female bovine reproductive tract secretions contained GAG and the concentrations and composition of those GAG varied with different regions and stages of the estrous cycle. This study was designed to evaluate the types of GAG found in reproductive tract secretions of ewes at different stages of t...

The objective of the study was to determine the effect of different bovine gamete coincubation times on fertilization and embryo development performance. In vitro matured COCs were co-incubated with sperm at a concentration of 1.5 × 10(6) spermatozoa/ml in TALP medium for 3 hours (T 3, n = 362), 6 hours (T 6, n = 358), or 18 hours (T 18, n = 350). At the end of the coincubation period COCs from...

In this study, buffalo spermatozoa were in-vitro capacitated and acrosome reacted using modified Tyrode’s bicarbonatebuffered medium (sp-TALP media). Lipids were extracted from freshly ejaculated, in-vitro capacitated and acrosome reacted buffalo spermatozoa. The total lipid content of freshly ejaculated spermatozoa (171.5±2.90 μg/108 cells), capacitated spermatozoa (128.2±3.29 μg/108 cells) an...

This paper describes our Georeferencing approaches, experiments, and results at the MediaEval 2014 Placing Task evaluation. The task consists of predicting the most probable geographical coordinates of Flickr images and videos using its visual, audio and metadata associated features. Our approaches used only Flickr users textual metadata annotations and tagsets. We used four approaches for this...