نتایج جستجو برای: synthetic promoter

تعداد نتایج: 228934  

2016
Pricila Hauk Kristina Stephens Ryan Mckay Chelsea Ryan Virgile Hana Ueda Marc Ostermeier Kyoung-Seok Ryu Herman O Sintim William E Bentley

Quorum sensing (QS) regulates many natural phenotypes (e.q. virulence, biofilm formation, antibiotic resistance), and its components, when incorporated into synthetic genetic circuits, enable user-directed phenotypes. We created a library of Escherichia coli lsr operon promoters using error-prone PCR (ePCR) and selected for promoters that provided E. coli with higher tetracycline resistance ove...

Journal: :The Plant journal : for cell and molecular biology 2005
Stephen Rutherford Federica Brandizzi Helen Townley Judith Craft Yibing Wang Ian Jepson Alberto Martinez Ian Moore

The synthetic transcription factor LhG4 has been used in numerous mis-expression studies in plants. We show that the sequence encoding the LhG4 activation domain, derived from Saccharomyces cerevisiae GAL4, contains several cryptic polyadenylation signals in Arabidopsis. The GAL4-derived sequence was modified according to preferred Arabidopsis codon usage, generating LhG4AtO which was faithfull...

2015
Rui Wang Menglin Zhu Rongjian Ye Zuoxiong Liu Fei Zhou Hao Chen Yongjun Lin

As an important part of synthetic biology, synthetic promoter has gradually become a hotspot in current biology. The purposes of the present study were to synthesize green tissue-specific promoters and to discover green tissue-specific cis-elements. We first assembled several regulatory sequences related to tissue-specific expression in different combinations, aiming to obtain novel green tissu...

Journal: :Journal of virology 2013
Devis Sinani Ethan Cordes Aspen Workman Prasanth Thunuguntia Clinton Jones

Alphaherpesvirinae family members can reactivate from latency following stress. The synthetic corticosteroid dexamethasone induces certain cellular transcription factors in murine and bovine trigeminal ganglionic neurons. Three dexamethasone-induced transcription factors, Krüppel-like factor 15, Slug, and SPDEF, stimulated the herpes simplex virus type 1-infected cell protein 0 (ICP0) promoter ...

Journal: :Molecular pharmacology 1999
G Siemann R Blume D Grapentin E Oetjen M Schwaninger W Knepel

The immunosuppressants cyclosporin A and FK506 (tacrolimus) can block the phosphatase calcineurin, thereby inhibiting gene transcription directed by the cyclic AMP (cAMP)- and calcium-responsive transcription factor, cAMP response element (CRE)-binding protein, and its binding site, CRE, in various cell lines. This action is a novel molecular mechanism of cyclosporin A and FK506 action. Because...

Journal: :The Journal of biological chemistry 2008
Olivier LaRochelle Simon Labbé Jean-François Harrisson Carl Simard Véronique Tremblay Geneviève St-Gelais Manjapra V Govindan Carl Séguin

Metal activation of metallothionein (MT) gene transcription is dependent on the presence of metal regulatory elements (MREs), which are present in five non-identical copies (MREa through MREe) in the promoter of the mouse MT-1 gene and on the capacity of metal transcription factor-1 (MTF-1) to bind to the MREs in the presence of zinc. We detected a protein, distinct from MTF-1, specifically bin...

Journal: :Virology 2015
Insun Kook Caitlin Henley Florencia Meyer Federico G Hoffmann Clinton Jones

The primary site for life-long latency of bovine herpesvirus 1 (BHV-1) is sensory neurons. The synthetic corticosteroid dexamethasone consistently induces reactivation from latency; however the mechanism by which corticosteroids mediate reactivation is unclear. In this study, we demonstrate for the first time that dexamethasone stimulates productive infection, in part, because the BHV-1 genome ...

Journal: :Molecular Systems Biology 2007
Robert Sidney Cox Michael G Surette Michael B Elowitz

Promoters control the expression of genes in response to one or more transcription factors (TFs). The architecture of a promoter is the arrangement and type of binding sites within it. To understand natural genetic circuits and to design promoters for synthetic biology, it is essential to understand the relationship between promoter function and architecture. We constructed a combinatorial libr...

2014
Wu Xu Xianzhen Jiang

Transl Androl Urol, Vol 3, Suppl 1 September 2014 www.amepc.org/tau © Translational Andrology and Urology. All rights reserved. effective promoter component can help us overcome these limitations. Methods: Here, we transfected the artificially hTERT promoter sequence into cancer cells and kept the specificity of the promoter. Then we used the Dual luciferase reporter gene system to prove the ar...

2015
Ling Li Chun-li Zhang Lei Kang Rong-Fu Wang Ping Yan Qian Zhao Lei Yin Feng-qin Guo

AIM To investigate the enhancing effect of radionuclide therapy by the therapeutic gene placed under the control of radio-responsive promoter. METHODS The recombinant lentivirus E8-codA-GFP, including a synthetic radiation-sensitive promoter E8, cytosine deaminase (CD) gene, and green fluorescent protein gene, was constructed. The gene expression activated by (125)I radiation was assessed by ...

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