The realization that nuclear distribution of DNA, RNA, and proteins differs between cell types and developmental stages suggests that nuclear organization serves regulatory functions. Understanding the logic of nuclear architecture and how it contributes to differentiation and cell fate commitment remains challenging. Here, we use soft X-ray tomography (SXT) to image chromatin organization, dis...

We correlate the localization of silver nanoparticles inside cells with respect to the cellular architecture with the molecular information in the vicinity of the particle surface by combining nanoscale 3D cryo-soft X-ray tomography (cryo-SXT) with surface-enhanced Raman scattering (SERS). The interaction of the silver nanoparticle surface with small molecules and biopolymers was monitored by S...

Data from the Y ohkoh Soft X-ray Telescope (SXT) and Bragg Crystal Spectrometer (BCS) are used to obtain the di†erential emission measure (DEM) of solar Ñare soft X-ray plasma. The maximum entropy method is used to obtain the DEM for 80 Ñares observed by SXT and BCS from 1991 October to 1992 November. We Ðnd that it is often difficult to describe the DEM using a monotonically decreasing functio...

We use Hinode observations to study coronal structures about to flare, based on their apparent footpoints as a guide to identification. The high resolution and excellent stability of the Hinode observations makes the identifications much more precise than those done with the soft X-ray telescope (SXT) on board Yohkoh. The physical conditions in the coronal structure about to flare are important...

OBJECTIVES Vibrio cholerae O1 from a 2006 outbreak in Accra were commonly resistant to multiple antimicrobials and, in particular, to trimethoprim/sulfamethoxazole, drugs commonly used in the treatment of cholera. We sought to determine the genetic basis for trimethoprim/sulfamethoxazole resistance in outbreak isolates. METHODS Twenty-seven isolates from the outbreak were screened by PCR and ...

Soft X-ray tomography (SXT) is becoming a powerful imaging technique to analyze eukaryotic whole cells close to their native state. Central to the analysis of the quality of SXT 3D reconstruction is the estimation of the spatial resolution and Depth of Field of the X-ray microscope. In turn, the characterization of the Modulation Transfer Function (MTF) of the optical system is key to calculate...

We present observations of four bright stars observed with the AstroSat Soft X-ray Telescope (SXT). Visible light from like these can leak through very thin filter in front CCD focal plane camera SXT and thus making extraction events difficult. Here, we show how to extract without contamination by visible light. The procedure applied here demonstrates reliable information be derived such cases....

In this study, it was aimed to contribute available epidemiological data and guide empirical treatment by determining the distribution antibiotic susceptibility of pathogenic microorganisms isolated from wound samples sent microbiology laboratory our hospital. The agents infection between 02.01.2017 20.07.2020 were retrospectively analyzed. grown identified conventional microbiological methods ...

Photobacterium damselae subsp. piscicida is a bacterial fish pathogen that causes a disease known as pasteurellosis. Two transferable multiple-drug resistance (R) plasmids, pP99-018 (carrying resistance to kanamycin, chloramphenicol, tetracycline, and sulfonamide) and pP91278 (carrying resistance to tetracycline, trimethoprim, and sulfonamide), isolated from P. damselae subsp. piscicida strains...