Non-covalent molecular imprinting of poly(allylamine hydrochloride) (PAA HCl) with glucose phosphate mono-sodium salt produced molecularly imprinted polymer (MIP) hydrogels capable of quantitative, isomerically specific binding of glucose. By ionic association of a template molecule, glucose phosphate mono-sodium salt, to the polymer prior to covalent crosslinking, MIP hydrogels were created wi...

Molecularly imprinted polymers (MIPs) were fabricated on glass slides with a "sandwich" technique giving ~20 µm thick films. Methanol/water as a solvent, and polyethyleneglycol and polyvinylacetate as solvent modifiers, were used to give a porous morphology, which was studied with scanning electron microscopy and gravimetric analysis. Various MIPs were synthesized through non-covalent imprintin...

Self-assembly and molecular imprinting technologies are very attractive technologies for the development of artificial recognition systems and provide chemical recognition based on need and not happenstance. In this paper, we employed a b-cyclodextrin derivative surface acoustic wave (SAW) chemical sensor for detecting the chemical warfare agents (CWAs) sarin (O-Isoprophyl methylphosphonofluori...

A universal label-free detection of bioanalytes can be performed with biomimetic quartz crystal microbalance (QCM) coatings prepared by imprinting strategies. Bulk imprinting was used to detect the endocrine disrupting chemicals (EDCs) known as estradiols. The estrogen 17β-estradiol is one of the most potent EDCs, even at very low concentrations. A highly sensitive, selective and robust QCM sen...

Self-assembly and molecular imprinting technologies are very attractive technologies for the development of artificial recognition systems and provide chemical recognition based on need and not happenstance. In this paper, we employed a β-cyclodextrin derivative surface acoustic wave (SAW) chemical sensor for detecting the chemical warfare agents (CWAs) sarin (O-Isoprophyl methylphosphonofluori...

To increase our understanding of imprinted genes in swine, we carried out a comprehensive analysis of this gene family using two complementary approaches: expression and phenotypic profiling of parthenogenetic fetuses, and analysis of imprinting by pyrosequencing. The parthenote placenta and fetus were smaller than those of controls but had no obvious morphological differences at Day 28 of gest...

The development of gene targeting approaches has had a tremendous impact on the functional analysis of the mouse genome. A specific application of this technique has been the adaptation of the bacteriophage P1 Cre/loxP site-specific recombinase system which allows for the precise recombination between two loxP sites, resulting in deletion or inversion of the intervening sequences. Because of th...

Nano-imprint lithography possesses the advantages of high throughput, sub-10-nm feature and low cost. In NIL, the mold filling is subjected to the applied imprinting pressure, temperature and time. Incomplete mold filling causes a detrimental effect on the final imprinted pattern dimensions. The monitoring system of imprinting is essential to control the imprinting parameters properly. Up to no...

In mammals, both parental genomes are essential for normal ontogeny because epigenetic modifications imposed in the parents' gametes lead to parent-of-origin specific gene expression in their offspring. These phenomena are referred to as genomic imprinting. It has been shown that maternal imprinting is established during oocyte growth, lack of maternal imprinting in zygotes leads to early embry...