An experimental approach for producing relative charge density maps of biological surfaces using the atomic force microscope is presented. This approach, called D minus D (D-D) mapping, uses isoforce surfaces collected at different salt concentrations to remove topography and isolate electrostatic contributions to the tip-sample interaction force. This approach is quantitative for surface poten...

The behavior of a polyelectrolyte adsorbed on a charged substrate of high-dielectric constant is studied by both Monte-Carlo simulation and analytical methods. It is found that in a low enough ionic strength medium, the adsorption transition is first-order where the substrate surface charge still keeps repulsive. The monomer density at the adsorbed surface is identified as the order parameter. ...

Ethanol introduced into the organism undergoes rapid metabolism to acetaldehyde and then to acetic acid. The process is accompanied by formation of reactive oxygen species (ROS), which damage mainly lipids of membrane cells. The effects of ROS can be neutralized by administering preparations with antioxidant properties. The natural preparations of this kind are teas.This paper reports data on t...

The surface charge of isolated apolipoprotein A-I, apo-A-II, apo-C-II, and apo-C-III2 as well as of liposomes from synthetic highly purified phospholipids and isolated very low density lipoprotein (VLDL) particles from different donors (n = 35) was determined by polyelectrolyte titration. The particle size of apolipoproteins was evaluated from their molecular weight and specific volume, while t...

Optimization of surface exposed charge-charge interactions in the native state has emerged as an effective means to enhance protein stability; but the effect of electrostatic interactions on the kinetics of protein folding is not well understood. To investigate the kinetic consequences of surface charge optimization, we characterized the folding kinetics of a Fyn SH3 domain variant containing f...

A simple statistical mechanical approach is applied to calculate the profile of the density of the number of particles and the profile of the electrostatic potential of an electric double layer formed by a charged cylindrical surface in contact with electrolyte solution. The finite size of particles constituting the electrolyte solution is considered by including the excluded volume effect with...

the transformation of biochemical information into a physical or chemical signal is the basic idea behind a biosensor. the efficient detection of charged biomolecules by biosensor with appropriate device has caught tremendous research interest in the present decade. the present work is related to the simulation study of the performance of a functionalized surface of a biologically sensitive fie...

This work considers the adhesion of cells to a nanorough titanium implant surface with sharp edges. The basic assumption was that the attraction between the negatively charged titanium surface and a negatively charged osteoblast is mediated by charged proteins with a distinctive quadrupolar internal charge distribution. Similarly, cation-mediated attraction between fibronectin molecules and the...

The effective surface charge obtained by fitting the Poisson-Boltzmann approximation to measured data is related to the actual surface charge. Evaluation of the formally exact expression, which depends upon the wall-ion direct correlation function, gives a precise measure of the amount of ion condensation in the planar double layer. Results are presented for 0.001, 0.01, and 0.1 M monovalent an...

Multidielectric polyethylene is a material that is generally employed as insulation for  the HVDC isolations. In this paper, the influence of temperature on space charge dynamics has been studied, low-density polyethylene (LDPE) and Fluorinated Ethylene Propylene (FEP) sandwiched between two electrodes were subjected to voltage application of 5kV (14.3 kV/mm) for extended duration of time ...