The cDNA of tomato chloroplastic Cu/Zn-superoxide dismutase was used to construct transgenic tobacco (Nicotiana plumbaginifolia). It was found that the gene expression of Cu/Zn-superoxide dismutase can constitutively be reduced in the transgenic tobacco plants because of double-stranded (dsRNA) expressed in the form of intronspliced hairpin structures and antisense suppression. Furthermore, the...

Previous studies demonstrated a significant reduction of ultimate infarct size in the canine heart by the combined administration of superoxide dismutase plus catalase. This study was performed to assess the independent effects of each enzyme on ultimate infarct size due to ischemia/reperfusion. Dogs received 2-hour infusions of superoxide dismutase, catalase, or albumin (controls) via the left...

Cytochrome c was reduced when superoxide was generated from xanthine oxidase in the presence of alloxan, and by the reaction of alloxan and with reduced glutathione. In each case, most of the reduction was inhibited by superoxide dismutase, but considerably more enzyme was required than with superoxide alone. This indicates that the superoxide dismutase-inhibitible cytochrome c reduction was ma...

The activities of superoxide dismutase and glutathione peroxidase in the red cells of normal and acatalasemia bloods were compared. Superoxide dismutase activity in the red cells of Japanese acatalasemia blood was higher than in normal control, while glutathione peroxidase activity remained within normal limits. Cyanide sensitivity of superoxide dismutase in the red cells of acatalasemia blood ...

A simple and rapid method for the assay of superoxide dismutase in biological samples is described. Present method takes advantage of the inhibition of NADH-dependent-nitroblue tetrazolium reduction by the dismutase. Inhibition of the chromogen formation by superoxide dismutase was linear with increase in enzyme concentrations. The chromogen extract in butanol was stable even up to 48 hr. Super...

objective(s):the aim of this study was to evaluate the oxidative status and dna integrity in testes of wistar rat offspring exposed to omega-9 monounsaturated (mufa) at different times of late organogenesis. materials and methods:sixty female rats were divided into six groups of 10 animals. the first group served as control and received the drug vehicle, olive oil (1 ml/kg/day). the second, thi...

in our previous studies we have evaluated the role of antioxidants and trace elements in potentially malignant disorders and cancers of the oral cavity, taking into consideration the importance of antioxidants as biomarkers in cancer detection. we felt that other than evaluation, the correlation and interdependence that existed among antioxidants and trace elements require further evaluation in...