Dietrich, 1976): however, background staining is high and variable. Alcian Blue with 0.05 M-MgCI, provided effective labelling of all cartilage glycosaminoglycans, including trace amounts of hyaluronate. The hyaluronate did not form a complex with Alcian Blue either in the absence of MgCI, or at molarities greater than 0.1. In the described scheme, hyaluronate was resolved from chondroitin and ...

The changes in concentration of oestrone, oestradiol (-17alpha and -17beta), oestrone sulphate and the oestradiol sulphates have been measured in allantoic and amniotic fluids and in maternal peripheral plasma throughout gestation. Oestrone sulphate was the major oestrone present in all of the fluids. It was measurable in allantoic fluid before Day 52 and reached a peak concentration of 475 ng/...

Little is known about the origin of the neuroactive steroids dehydroepiandrosterone sulphate (DHEAS) and pregnenolone sulphate (PregS) in the brain or of their subsequent metabolism. Using rat brain perfusion in situ, we have found 3 H-PregS to enter more rapidly than 3 H-DHEAS and both to undergo extensive (> 50%) desulphation within 0.5 min of uptake. Enzyme activity for the steroid sulphatas...

A total of 834 individual aerosol particles were collected during October and November 2010 in urban Shanghai, China. Particles were sampled under different weather and air quality conditions. Morphologies, compositions and mixing states of carbonaceous aerosols were investigated by transmission electron microscopy (TEM) coupled with energy-dispersive X-ray (EDX). Structures of some particles w...

come across a medical man who avers that it is a useless salt of Quinine and that the only salt of anv use for injection is the Bisulpliate of Quinine, Now, from what I can remember of the Physiology of the Blood, I should have thought that the Bisulpliate was a much more foreign salt to the plasma than the Bihydrochloride. As far as I can remember the plasma contains Chlorides in abundance, bu...

The effect of all-trans retinoic acid on metastatic B16 melanoma lung colonization and synthesis and properties of glycosaminoglycans was examined. Injection of tumour cells, pretreated with 10(-6) M-retinoic acid or grown to low density, into the tail vein of syngeneic C57 mice produced significantly fewer pulmonary tumours compared to subconfluent control cells. By cochromatography of glycosa...

Nowadays, one of the most popular ways to get a more sustainable cement industry is using additions as cement replacement. However, there are many civil engineering applications in which the use of sustainable cements is not extended yet, such as special foundations, and particularly micropiles, even though the standards do not restrict the cement type to use. These elements are frequently expo...

Glycosaminoglycans and glycoproteins in the urine of 100 healthy, active, human subjects were examined by cellulose acetate electrophoresis and salt gradient, ion-exchange, column chromatography. The cetylpyridinium chloride (CPC) turbidity and uronic acid:creatinine ratio was also studied. Fractions were identified by electrophoretic mobility, staining reactions, susceptibility to enzyme diges...

The content of chlorides, nitrites, nitrates, phosphates and sulphates was used to classify 45 donkey's milk samples collected from different Italian regions. A method employing ion exchange chromatography with conductivity detector and chemical suppression was used. The quantitative results indicated phosphates (569.4-1304.4 mg kg(-1)) and chlorides (545.9-1757.9 mg kg(-1)) as being the most a...