The electron spin echo envelope modulation (ESEEM) investigation of the high-pH (hpH) form of sulfite oxidase (SO) and sulfite dehydrogenase (SDH) prepared in buffer enriched with H(2)(17)O reveals the presence of three types of exchangeable oxygen atoms at the molybdenum center. Two of these oxygen atoms belong to the equatorial OH ligand and the axial oxo ligand, and are characterized by (17)...

deficiency. It is not applicable to newborn or mass screening with use of ifiterpaper specimens, but is satisfactory for liquid urine screening. In one variant of sulfite oxidase deficiency, due to a defect of its molybdate cofactor metabolism, the concentration of uric acid in serum is decreased. Thus, an unexplained low serum uric acid, especially in a young patient with neurologic abnormalit...

Recently, the nanotechnology industry has seen a growing interest in integrating silver nanoparticles (AgNPs) into agricultural products, which increases soil exposure to these particles. This demands an investigation effect of AgNPs on health. Changes enzyme activities upon can serve as early indicators any adverse effects that particles may have quality. study aimed determine AgNP size, conce...

Representative examples of the various classes of flavoproteins have been converted to their apoprotein forms and the native flavin replaced by 8-mercapto-FMN or 8-mercapto-FAD. The spectral and catalytic properties of the modified enzymes are characteristically different from one group to another; the results suggest that flavin interactions at positions N(1) or N(5) of the flavin chromophore ...