Three antibodies reacting with corneal keratan sulfate proteoglycan were used to detect antigenically related molecules in 11 bovine and 13 embryonic chick tissues. Two monoclonal antibodies recognized sulfated epitopes on the keratan sulfate chain and a polyclonal antibody bound antigenic sites on the core protein of corneal keratan sulfate proteoglycan. Competitive immunoassay detected core p...

We show that aqueous sodium sulfate solutions exhibit an unrealistically large degree of ion pairing and clustering when modeled using nonpolarizable force fields, with clusters resembling precipitate readily forming in a 0.5 m solution at ambient conditions. This aggregation behavior was found to be persistent in nonpolarizable water for a range of parameters of the sulfate anion. In contrast,...

Fractionation of rat mammary gland extracts by ion eschange chromatography has led to the isolation of a new low molecular weight derivative of neuraminic acid. This compound appears to be a sulfuric ester of neuramin-lactose (1) and, therefore, the name neuramin-lactose sulfate is here proposed for it. Glands obtained from the first to the sixth da.y after delivery contained an average of 3.4 ...

One heat-modifiable protein of Escherichia coli outer membrane does not completely change to the high-temperature form in the presence of magnesium ion in sodium dodecyl sulfate solution. When the metal ion complexing reagents ethylenediaminetetraacetic acid, phosphate ion, hydroxyl ion, or the competitive cations Zn2+ or Ca2+ are added to the sodium dodecyl sulfate-solubilized sample of outer ...

In the title compound [Ca(C(12)H(25)O(4)S)(2)](n), the unique Ca(II) ion lies on an inversion center and is coordinated in a slightly distorted octa-hedral environment by six O atoms from dodecyl sulfate anions. The crystal structure is based on hydro-carbon (dodecyl sulfate) layers which sandwich the Ca(II) ions. Within the layers, the hydro-carbon zigzag chains are parallel to one another and...

Chondroitin sulfate (CS) and dermatan sulfate (DS) glycosaminoglycans display variability of sulfation in their constituent disaccharide repeats during chain elongation. Since a large proportion of the extracellular matrix of the central nervous system (CNS) is composed of proteoglycans, CS/DS disaccharide degree and profile of sulfation play important roles in the functional diversity of neuro...

Hcpatic a nd splenic amyloidosis was experimentally induced by casein injections in BA LB; C mice and examincd biochemically for glycosaminoglycan constituents. Heparan sulfate and dermatan sulfate were shown to be major components by clectrophoresis on cellulose acetate membrane. It was demonstrated from the studies of DEAE-eellulose ion-exchanging chromatography that the amount of highly sulf...