17alpha-Ethinylestradiol (EE2), a synthetic and potent estrogen receptor agonist, is extensively metabolized in both intestine and liver and is largely excreted in bile and urine as the 3-O-sulfate (EE2-Sul) and 3-O-glucuronide. In the present study, EE2-Sul was evaluated as a substrate of various transporters known to be expressed in the kidney. Uptake studies were performed with human epithel...

C. K. Macgowan, S. Wu, R. Tessler, J. Belik, and A. Kassner Medical Biophysics, University of Toronto, Toronto, ON, Canada, Physiology and Experimental Medicine, Hospital for Sick Children, Toronto, ON, Canada, Pediatrics, Pontifcia Universidade Catolica do Rio Grande do Sul, Rio Grande do Sul, Brazil, Pediatrics, University of Toronto, Toronto, ON, Canada, Medical Imaging, University of Toront...

Organic anion transporting polypeptide (OATP) 1B1 and OATP1B3 are responsible for the hepatic uptake of organic anions. They share similar sequences and structures with 12 putative transmembrane domains (TMs). Their substrate specificities are very broad and overlap each other, whereas each transporter specifically recognizes certain substrates. Because the homology of the amino acid sequence i...

Spreading manure containing antibiotics in agriculture is assumed to stimulate the dissemination of antibiotic resistance in soil bacterial populations. Plant roots influencing the soil environment and its microflora by exudation of growth substrates might considerably increase this effect. In this study, the effects of manure from pigs treated with sulfadiazine (SDZ), here called SDZ manure, o...

We assessed the clonality of consecutive Escherichia coli isolates during the course of recurrent urinary tract infections (RUTI) in childhood in order to compare clonality with phenotypic antibiotic resistance patterns, the presence of integrons, and the presence of the sul1, sul2, and sul3 genes. Altogether, 78 urinary E. coli isolates from 27 children, who experienced recurrences during a 1-...

Bacteria colonizing the human intestine have a relevant role in the spread of antimicrobial resistance. We investigated the faecal carriage of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in healthy humans from Portugal and analyzed the distribution of sul genes and class 1 and 2 integrons. Faecal samples (n = 113) were recovered from healthy persons (North/Centre of Por...

igh-level carbapenem resistance in Pseudomonas aeruginosa s a recognized public health problem. Metallo-lactamase MBL) is one of the most common resistance mechanisms n these microorganisms. The gene blaSPM-1, which is the ost frequent metallo-lactamase gene in Brazil, was first escribed in 20021 and has been found in different regions.2 ntil now there has been no record of carbapenemase enzyme...

Universidade Federal do Rio Grande do Sul.

This paper reports on the Phytoseiidae from an Araucaria forest in the State of Rio Grande do Sul, describing four new species, namely Transeius kroeffis n. sp., Typhlodromalus araucariae n. sp., Typhlodromips pompeui n. sp. and Typhlodromips salvadorii n. sp.. Iphiseiodes moraesi Ferla & Silva, Neoseiulus tunus (DeLeon), Typhlodromips japi Lofego, Demite & Feres, Typhlodromips pallinii Gonçalv...

First submission on 05/09/13; last submission on 06/11/13; accepted for publication on 06/11/13; published on 20/02/14 1. Post-doctorate in Cardiovascular Pathology from Instituto de Cardiologia do Rio Grande do Sul; assistant professor of Histology and Pathology at Universidade Federal do Rio Grande do Sul (UFRGS). 2. Master’s degree in Pathology from Universidade Federal de Ciências da Saúde ...