We attempted to determine the number of sperm cells required for genotyping of one microsatellite marker. The crude genomic DNA extracted from about 760 or more sperm cells gave sufficient quantity of PCR product using a 20 microl-scale PCR. We also studied the effects of non-ionic detergents on extraction of crude sperm genomic DNA. PCR products amplified with the crude sperm genomic DNA extra...

The idea of dimension-reduction without loss of information can be quite helpful for guiding the construction of summary plots in regression without requiring a pre-specified model. Focusing on the central subspace, we investigate such “sufficient” dimension-reduction in regressions with a binary response. Three existing methods, SIR and pHd and SAVE, and one new method DOC are studied for thei...

Chen et al. (2010) propose a unified method – coordinate-independent sparse estimation (CISE) – that is able to simultaneously achieve sparse sufficient dimension reduction and screen out irrelevant and redundant variables efficiently. However, its attractive features depend on appropriate choice of the tuning parameter. In this note, we re-examine the Bayesian information criterion (BIC) in su...

thrombotic thrombocytopenic purpura (ttp) is a rare syndrome affecting multiple organs. there is no sufficient evidence regarding the clinical cardiac manifestations of ttp. nonetheless, pathologic cardiac involvement is quite frequent in acute ttp, which is predominantly manifested as myocardial necrosis due to coronary arteriolar microthrombosis. the present case report describes a 43-year-ol...

when the parties enter into a contract it is valid and binds the parties: that meams one of them cannot terminate the contract without an agreement; in this article we try to prove the base of this principle: in islamic law if the evidence of the sources are not sufficient we can accompany the effects of the contract if it is unconditional and absolute in islamic law it is known as esteshaab.

Recent activation and electrophysiological studies have demonstrated that sound recognition and localization are processed in two distinct cortical networks that are each present in both hemispheres. Sound recognition and/or localization may be, however, disrupted by purely unilateral damage, suggesting that processing within one hemisphere may not be sufficient or may be disturbed by the contr...