Background: Eptifibatide (Integrilin) is an intravenous (IV) peptide drug that selectively inhibits ligand binding to the platelet GP IIb/IIIa receptor. It is an efficient peptide drug, however has a short half-life. Therefore, antithrombotic agents like eptifibatide are required to become improved with a protected and targeted delivery system such as using nano-liposomes to the site of thrombu...

In the present study we attempted to correlate the structure and function of the cry11a (72 kDa) and cry4a (135 kDa) proteins of Bacillus thuringiensis var israelensis. Homology modeling and secondary structure predictions were done to locate most probable regions for finding helices or strands in these proteins. The JPRED (JPRED consensus secondary structure prediction server) secondary struct...

a new biological active hexapeptide of c-terminal of nocistatin, contains glu-gln-lys-gln-leu-gln sequence was synthesized according to solid phase peptide synthesis on the surface of 2-chloro tritylchloride resin and using fmoc-protected amino acids in the presence of tbtu (o-(benzotriazol-1-yl)-n,n,n',n'-tetramethyl uranium tetrafluoroborate) as a coupling reagent. then, amidation o...

background: acute respiratory distress (ard) is a critical respiratory failure due to lung injury of neonates leading to the clinical appearance of poor lung compliance. the aimed of the study was to evaluate the diagnostic values in differentiating respiratory from heart diseases with using of n-terminal pro brain natriuretic peptide (nt-pro bnp) and atrial natriuretic peptide(anp) in neonates...

random peptide libraries (rpl) displayed on the surface of filamentous bacteriophages have been extensively used as a tool to map epitopes or to identify antigenic mimics (mimotpoes) of disease-specific monoclonal antibodies or polyclonal sera. these rpl are engineered by the insertion of degenerate oligonucleotides, encoding a specific number of random amino acids, in frame with a bacteriophag...

objective(s):consumption of high-fat foods is one of the major causes of obesity. physical exercise is a strategy used to counteract obesity. the aim of this study was to investigate the effect of eight weeks endurance training and high-fat diet (hfd) on appetite-regulating hormones in rat plasma. materials and methods:twenty eight male wistar rats were randomly divided into four groups: contro...

Recent X-ray crystallographic analyses have demonstrated that the receptor-binding (B) subunits of Shiga toxin (STX) are arranged as a doughnut-shaped pentamer. The C terminus of the enzymatic (A) subunit presumably penetrates the nonpolar pore of the STX B pentamer, and the holotoxin is stabilized by noncovalent interactions between the polypeptides. We identified a stretch of nine nonpolar am...

A Shiga toxin (Stx)-encoding temperate bacteriophage of Shigella sonnei strain CB7888 was investigated for its morphology, DNA similarity, host range, and lysogenization in Shigella and Escherichia coli strains. Phage 7888 formed plaques on a broad spectrum of Shigella strains belonging to different species and serotypes, including Stx-producing Shigella dysenteriae type 1. With E. coli, only s...

Shiga and the Shiga-like toxins are related protein toxins produced by Shigella dysenteriae and certain strains of Escherichia coli. These toxins are composed of two non-covalently attached, modular parts: the A moiety (StxA) containing the enzymatically active A1 fragment, and the non-toxic, pentameric binding moiety (StxB). Stx binds specifically to the glycosphingolipid globotriaosylceramide...