In this study, we evaluated suitable selected markers and optimized transformation protocols to develop a new genetic transformation methodology for DHA-producing Crypthecodinium cohnii. Additionally, ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO), potentially involved in CO2 fixation under autotrophic conditions, was selected as the target for construction of a gene knockdown mutant...

The aerial parts of H. perforatum subsp. perforatum and H. perforatum subsp. angustifolium were investigated for their chemical composition and antimicrobial activity. Spectrophotometric analysis indicated that H. perforatum subsp. perforatum is richer in flavonoids and tannins than the other subspecies. HPLC analysis confirmed the higher yield of flavonoids in H. perforatum subsp. perforatum a...

OBJECTIVES To assess the prevalence of Ureaplasma urealyticum and Mycoplasma hominis in women experiencing chronic urinary symptoms. METHODS Urine, vaginal, and urethral samples obtained from 153 women presenting with chronic voiding symptoms were tested for the presence of pathogens including U. urealyticum and M. hominis. Patients with positive cultures for Mycoplasma were treated with a si...

Hyperammonemia syndrome is an often fatal complication of lung transplantation which has been recently associated with Ureaplasma infection. It has not been definitely established that Ureaplasma species can cause hyperammonemia. We established a novel immunocompromised murine model of Ureaplasma urealyticum infection and used it to confirm that U. urealyticum can cause hyperammonemia. Male C3H...

The API STAPH-IDENT system was compared with conventional methods for the identification of 14 Staphylococcus species. Conventional methods included the Kloos and Schleifer simplified scheme and DNA-DNA hybridization. The API STAPH-IDENT strip utilizes a battery of 10 miniaturized biochemical tests, including alkaline phosphatase, urease, beta-glucosidase, beta-glucuronidase, and beta-galactosi...

Ureaplasma urealyticum has been divided into 14 serovars. Recently, subdivision of U. urealyticum into two species has been proposed: U. parvum (previously U. urealyticum parvo biovar), comprising four serovars (1, 3, 6, 14) and U. urealyticum (previously U. urealyticum T-960 biovar), 10 serovars (2, 4, 5, 7-13). The multiple-banded antigen (MBA) genes of these species contain both species and ...

The Genus Staphylococcus belong to the family Staphylococcaceae which comprise of 53 valid species. These are normal flora mucocutaneous membranes mammals and usually felines. nepalensis is one member genus show close resemblance cohnii, gallinarum succinus. Thus, phenotypic tests not sufficient characterize them sufficiently, molecular intervention required for their correct identification. In rec...

SUMMARY Dinoflagellates genomes are large (up to 200 pg) and are encoded in histoneless chromosomes that are quasi-permanently condensed and circular. This unique combination of chromosomal characteristics presents additional topological and cell cycle control problems for a eukaryotic cell, potentially requiring novel topoisomerase activities. The heterotrophic dinoflagellate Crypthecodinium c...

We evaluated the clinical usefulness of species identification of blood isolates of coagulase-negative staphylococci as a predictor of the clinical significance of the isolates. In addition, we compared results of species identification obtained with MicroScan Rapid Gram-Positive Identification panels and Dried Overnight (Conventional) Gram-Positive Identification panels with those obtained by ...