نتایج جستجو برای: stalling

تعداد نتایج: 1301  

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2013
Anders R Clausen Michael S Murray Andrew R Passer Lars C Pedersen Thomas A Kunkel

Ribonucleotides are frequently incorporated into DNA during replication, they are normally removed, and failure to remove them results in replication stress. This stress correlates with DNA polymerase (Pol) stalling during bypass of ribonucleotides in DNA templates. Here we demonstrate that stalling by yeast replicative Pols δ and ε increases as the number of consecutive template ribonucleotide...

Journal: :The Journal of Cell Biology 1995
S K Lyman R Schekman

To clarify the roles of Kar2p (BiP) and Sec63p in translocation across the ER membrane in Saccharomyces cerevisiae, we have utilized mutant alleles of the essential genes that encode these proteins: kar2-203 and sec63-1. Sanders et al. (Sanders, S. L., K. M. Whitfield, J. P. Vogel, M. D. Rose, and R. W. Schekman. 1992. Cell. 69:353-365) showed that the translocation defect of the kar2-203 mutan...

2013
Justin D. Lormand Noah Buncher Connor T. Murphy Parminder Kaur Marietta Y. Lee Peter Burgers Hong Wang Thomas A. Kunkel Patricia L. Opresko

Previous evidence indicates that telomeres resemble common fragile sites and present a challenge for DNA replication. The precise impediments to replication fork progression at telomeric TTAGGG repeats are unknown, but are proposed to include G-quadruplexes (G4) on the G-rich strand. Here we examined DNA synthesis and progression by the replicative DNA polymerase d/proliferating cell nuclear an...

2013
Karen J. Ouyang Mary K. Yagle Michael J. Matunis Nathan A. Ellis

Polymerase stalling results in uncoupling of DNA polymerase and the replicative helicase, which generates single-stranded DNA (ssDNA). After stalling, RAD51 accumulates at stalled replication forks to stabilize the fork and to repair by homologous recombination (HR) double-strand breaks (DSBs) that accumulate there. We showed recently that SUMO modification of the BLM helicase is required in or...

2011
Yongguang Tao Shuang Liu Victorino Briones Theresa M. Geiman Kathrin Muegge

Inactivation of tumor suppressor genes plays an important role in tumorigenesis, and epigenetic modifications such as DNA methylation are frequently associated with transcriptional repression. Here, we show that gene silencing at selected genes with signs of DNA hypermethylation in breast cancer cells involves Pol II stalling. We studied several repressed genes with DNA hypermethylation within ...

2011
Shashi Bhushan Thomas Hoffmann Birgit Seidelt Jens Frauenfeld Thorsten Mielke Otto Berninghausen Daniel N. Wilson Roland Beckmann

As nascent polypeptide chains are synthesized, they pass through a tunnel in the large ribosomal subunit. Interaction between specific nascent chains and the ribosomal tunnel is used to induce translational stalling for the regulation of gene expression. One well-characterized example is the Escherichia coli SecM (secretion monitor) gene product, which induces stalling to up-regulate translatio...

2016
Ryo Yonashiro Erich B Tahara Mario H Bengtson Maria Khokhrina Holger Lorenz Kai-Chun Chen Yu Kigoshi-Tansho Jeffrey N Savas John R Yates Steve A Kay Elizabeth A Craig Axel Mogk Bernd Bukau Claudio A P Joazeiro

Ribosome stalling during translation can potentially be harmful, and is surveyed by a conserved quality control pathway that targets the associated mRNA and nascent polypeptide chain (NC). In this pathway, the ribosome-associated quality control (RQC) complex promotes the ubiquitylation and degradation of NCs remaining stalled in the 60S subunit. NC stalling is recognized by the Rqc2/Tae2 RQC s...

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