Surface plasmon resonance (SPR) sensing is an optical technique that allows real time detection of small changes in the physical properties, in particular in the refractive index, of a dielectric medium near a metal film surface. One way to increase the SPR signal shift is then to incorporate a substance possessing a strong dispersive refractive index in the range of the plasmon resonance band....

INTRODUCTION Various optical measurement techniques have been developed for the characterization of surface states during electrochemical processes. Surface plasmon resonance (SPR) spectroscopy has been used in combination with electrochemistry to investigate the electronic structure of metal-electrolyte interfaces [1]. We reported the current efficiency of refractive index changes that occurre...

Sepiapterin reductase (SPR) is known as an essential enzyme for the biosynthesis of tetrahydrobiopterin. SPR belongs to the short-chain dehydrogenase/reductase (SDR) family and also reduces various exogenous carbonyl compounds including phenylpropanedione. We found in the present study that phenylpropanedione decreased the rate of proliferation of PC12 cells and that this rate was further dimin...

Fragment-based screening has recently evolved into a promising strategy in drug discovery, and a range of biophysical methods can be employed for fragment library screening. Relevant approaches, such as X-ray, NMR and tethering are briefly introduced focussing on their suitability for fragment-based drug discovery. In particular the application of surface plasmon resonance (SPR) techniques to t...

We anticipate that surface plasmon resonance (SPR) technology will remain the gold standard in direct biomolecular interaction sensing during the next decade. Although in the past only one company (Biacore AB) mainly dominated the market (B90%) of high-quality SPR systems including optics, liquid handling and sensor chips, new players and new trends can be identified. As highlighted in Chapter ...

We present the optimization of a new material structure used as transducer in a MagnetoOptical Surface Plasmon Resonance (MOSPR) biosensor. The MOSPR sensor is a modification of the standard Surface Plasmon Resonance (SPR) biosensor, which arises from the introduction of a ferromagnetic metal layer with Magneto-Optical (MO) activity together with the usual gold layer of SPR biosensors. Such com...

This paper reports the design analysis of a microfabricatable mid-infrared (mid-IR) surface plasmon resonance (SPR) sensor platform. The proposed platform has periodic heavily doped profiles implanted into intrinsic silicon and a thin gold layer deposited on top, making a physically flat grating SPR coupler. A rigorous coupled-wave analysis was conducted to prove the design feasibility, charact...

Surface plasmon resonance (SPR) is a well-established optical biosensor technology with many proven applications in the study of molecular interactions as well as in surface and material science. SPR is usually applied in the label-free mode which may be advantageous in cases where the presence of a label may potentially interfere with the studied interactions per se. However, the fundamental c...

We present an experimental setup for the simultaneous measurement of surface plasmon resonance (SPR) and x-ray absorption spectroscopy (XAS) on metallic thin films at a synchrotron beamline. The system allows measuring in situ and in real time the effect of x-ray irradiation on the SPR curves to explore the interaction of x-rays with matter. It is also possible to record XAS spectra while excit...

We design terahertz (THz) surface-plasmonresonance (SPR) sensors using a ferroelectric polyvinylidene fluoride (PVDF) thin layer for biological sensing. The reflectivity properties based on SPR are described using transfer matrix method (TMM) and numerically simulated using finite-difference time domain (FDTD) method. The sensing characteristics of the structure are systematically analyzed thro...