The sphingosine 1-phosphate receptor 1 (S1PR1) is one of five G protein-coupled receptors activated by the lipid sphingosine 1-phosphate (S1P). Stimulation of S1PR1 by binding S1P or the synthetic agonist FTY720P results in rapid desensitization, associated in part with depletion of receptor from the cell surface. We report here combining spinning disc confocal fluorescence microscopy and flow ...

Spinning disc confocal microscopy of LLCPK1 cells expressing GFP-tubulin was used to demonstrate that microtubules (MTs) rapidly elongate to the cell cortex after anaphase onset. Concurrently, individual MTs are released from the centrosome and the centrosome fragments into clusters of MTs. Using cells expressing photoactivatable GFP-tubulin to mark centrosomal MT minus ends, a sevenfold increa...

We perform simultaneous interferometric and fluorescent detection of molecular protein layers on a BioCD. The 488 nm excitation wavelength of fluorescein also provides the interferometric detection channel that operates in a common-path in-line configuration in the condition of phase quadrature set by a thermal oxide on silicon. The simultaneous acquisition of both channels enables a direct cor...

We consider the steady laminar annular spread of a thin liquid film generated by a circular jet impinging perpendicularly in direction of gravity on the centre of a horizontally rotating disc (figure 1). The individual flow regimes arising due to the largeness of the Reynolds number formed with the radius of the jet, its slenderness, and the relative magnitude of the centrifugal force measured ...

In this paper, a DEM (Discrete Element Method) model is presented and a series of computer experiments is analyzed and compared to experimental validations. Also, the simulations are compared to experimental results. The model uses a 3 parameter contact force to calculate impact between particles and other object such as vanes. The components of the contact forces are typically modeled in terms...

Electrocentrifugal spinning is a recently developed spinning system whose performance is still under investigation by researchers. In this study the process of jet formation in electrocentrifugal spinning is explored and compared to the same process in electrospinning and centrifuge spinning. The results show that the incorporation of the electrical and the centrifugal forces in the electrocent...

This study demonstrates the fabrication of κ-carrageenan fibers by a wet-spinning method and discusses three important spinning parameters: coagulation bath composition, spinning rate and post-spinning mechanical drawing. The as-spun fiber diameter decreased with KCl and ethanol concentration in the coagulation bath. In general, the ultimate tensile stress and elongation at break both increased...

We present a time-dependent model of the interaction between a neutron star magnetosphere and a thin (Shakura-Sunyaev) accretion disk, where the extent of the magnetosphere is determined by balancing outward diffusion and inward advection of the stellar magnetic field at the inner edge of the disc. The nature of the equilibria available to the system is governed by the magnetic Prandtl number P...

Minute-to-minute control of the release of insulin by pancreatic beta-cells in response to glucose or other stimuli requires the precise delivery of large dense-core vesicles to the plasma membrane and regulated exocytosis. At present, the precise spatial organization at the cell surface and the nature of these events ('transient' versus 'full fusion') are debated. In order to monitor secretory...

Recent X-ray observations have shown evidence for exceptionally broad and skewed iron Kα emission lines from several accreting black hole systems. The lines are assumed to be due to fluorescence of the accretion disk illuminated by a surrounding corona and require a steep emissivity profile increasing in to the innermost radius. This appears to question both standard accretion disc theory and t...