Amperometric biosensors using Pseudomonas putida cells as a bioelement were developed for 2,4-dichloro phenoxy acetic acid (2,4-D). After the adaptation process of Pseudomonas putida to 2,4-D, cells were immobilized onto the screen printed graphite electrodes (SPG) as well as Clark oxygen probe by gelatin and glutaraldehyde. Optimum pH, temperature, and stability of the biosensor were investiga...

Introduction Central venous vascular tears constitute the most lethal complication encountered during transvenous lead extraction. Although they are infrequent, the case fatality rate for these events has been reported as high as 63% owing to rapid exsanguination. Of those who survive these encounters, significant morbidity often follows. In this report, we describe a case of symmetrical periph...

Introduction Stroke is a major cause for disability, death and health care expenditure. To date, the most common treatment for ischemic stroke is tissue plasminogen activator (tPA). However, despite its widespread use, this treatment has some limitations, especially its short therapeutic window. Therefore other therapeutic materials and devices should be developed. Electrical stimulation of the...

The role of Fos proteins in the regulation of germ cell progression during spermatogenesis has been studied in the frog, Rana esculenta. A peculiarity of this animal model is the finding of Fos in cytoplasmic compartment of primary spermatogonia during the resting period of the annual reproductive cycle. Interestingly, Fos is localized in the nuclear compartment when spermatogenesis resumes. Us...

Abstract The Steiner tree problem in graphs (SPG) is one of the most studied problems combinatorial optimization. In past 10 years, there have been significant advances concerning approximation and complexity SPG. However, state art (practical) exact solution SPG has remained largely unchallenged for almost 20 years. While DIMACS Challenge 2014 PACE 2018 brought renewed interest into problems, ...

Missense mutations in the human PLP1 gene lead to dysmyelinating diseases with a broad range of clinical severity, ranging from severe Pelizaeus-Merzbacher disease (PMD) to milder spastic paraplegia type 2 (SPG-2). The molecular pathology has been generally attributed to endoplasmic reticulum (ER) retention of misfolded proteolipid protein (PLP) (and its splice isoform DM20) and induction of th...

OBJECTIVES We sought to assess the effect of successful stenting on the Doppler profile of aortic coarctation and to identify echocardiographic indexes that could be used for follow-up of such patients. BACKGROUND Doppler echocardiography demonstrates characteristic flow patterns in significant aortic coarctation. METHODS We undertook retrospective echocardiographic analyses before and at s...

Evidence has been accumulated indicating that GnRH-like peptides are present in a variety of extrabrain areas of mammalian and nonmammalian vertebrates. A pioneer study carried out in the frog, Rana esculenta, demonstrated that testicular GnRH induced spermatogonial proliferation. Recently, we have shown that in proliferating spermatogonia (SPG) of frogs, a change of localization of the oncopro...

Surface seawater in the South Pacific Gyre (SPG) is one of the cleanest oceanic environments on earth, and the photosynthetic primary production is extremely low. Despite the ecological significance of the largest aquatic desert on our planet, microbial community composition in the ultra-oligotrophic seawater remain largely unknown. In this study, we collected surface seawater along a southern ...