introduction: semen parameters and specially sperm morphology is used for the assessment of semen sample and for the prediction of the fertilization. however there is semen samples with normal sperm morphology but with hidden sperm chromatin anomalies. since sperm morphology and chromatin anomalies reduce the fertilization rat independently, the purpose of this study is to consider the relation...

This study was aimed to evaluate the effects of dietary n-6:n-3 ratio and Vitamin E on the membrane properties and motility characteristics of spermatozoa in boars. Forty Duroc boars were randomly distributed in a 2 × 2 factorial design with two n-6:n-3 ratios (14.4 and 6.6) and two Vitamin E levels (200 and 400 mg kg-1 ). During 16 weeks of treatment, fresh semen was collected at weeks 0, 8, 1...

objective: the aim of the present study was to evaluate three thawing rates on the post thaw motility, viability and chromatin structure of buffalo semen frozen in 0.5-ml straws. materials and methods: in this experimental study semen was collected with artificial vagina (42˚c) from four buffalo bulls.split pooled ejaculates (n=4) were extended at 37˚c with a bioxcell® extender. semen was coole...

Practical techniques for assessing semen quality in order to predict male fertility are still needed. The principal objective of this experiment was to evaluate routine laboratory evaluation and in vitro fertilization (IVF) techniques as predictors of relative boar fertility using a low-dose AI protocol. Nine boars were evaluated during a 6.5+/-1 mo period, beginning at 29-32 wk of age. Ejacula...

background: sperm dna fragmentation is known as an important cause of male infertility. the influence of sperm dna damage on reproductive potential has been subject of many studies indicating various results and remaining the subject controversial. in this study, we investigated differences of the semen parameters and intracytoplasmic sperm injection (icsi) outcome according to sperm dna fragme...

Mammalian sperm mitochondrial sheath harbors a number of enzymes that are involved in release of energy via oxidative phosphorilation of ATP. The present study was designed to detect the effect of differential sperm mitochondrial activity on the fertility potential and the association of sperm mitochondrial activity index (SMAI) with the sperm motility parameters for cryopreserved semen samples...

OBJECTIVE To evaluate and compare standard sperm parameters, lipid peroxidation of sperm plasma membranes, antioxidant capacity of seminal plasma, and sperm chromatin integrity in ejaculates from men whose partners have a history of recurrent pregnancy loss and from a control group of men with recent fertility. DESIGN Descriptive study. SETTING Reproduction Program, University of Antioquia,...

The purpose of this study was to evaluate the probable effects of leptin addition in different levels to the semen extender on sperm quality (motility and motility parameters, viability, sperm membrane integrity, and DNA damage). Semen specimens were evaluated immediately after leptin addition, equilibration time and after thawing the frozen semen. Five healthy buffalo bulls (5 ejaculates from ...

The selection of morphologically normal spermatozoa is critical to obtain high breeding performances in boar breeding farms and artificial insemination (AI) centers. Parameters for the selection of semen mainly include total sperm motility, concentration, and morphology. However, these primary parameters are often not reliable for discriminating between normal and abnormal, non-fertilizable spe...