BACKGROUND Sperm chromatin status and nuclear DNA damage can be detected using well-established assays. However, most techniques are time-consuming and/or involve elaborate protocols and equipment. We have recently developed a simple and fast method to monitor sperm chromatin status in field conditions using the Diff-Quik assay which is employed in fertility clinics to assess sperm morphology w...

background: one of the main laboratory tests for evaluation of infertility is semen analysis (sa). however additional tests may be suggested for further diagnosis of male fertility potentials. the seminal fluid contains sperms non-sperm cells and various types of lipids and glucose. objective: the objective of this cross-sectional study was to correlate the sperm parameters with concentrations ...

background: recently, motile sperm organelle morphology examination (msome) criteria as a new real time tool for evaluation of spermatozoa in intracytoplasmic sperm injection (icsi) cycles has been considered. objective: the aim was to investigate the predictive value of msome in in vitro fertilization (ivf) in comparison to icsi cycles and evaluation of the association between msome parameters...

Species with alternative reproductive strategies are characterized by discrete differences among males in suites of traits related to competition for fertilizations. Models predict sneaker males should allocate more resources to their ejaculates because they experience sperm competition more frequently and often occupy a disfavoured 'role' owing to subordinance in intramale competition and fema...

Background: Sports, hypothermia and hyperthermia as some stressors inhibit male reproductive functions and are associated with subfertility or infertility. We investigated whether water temperature and chronic swimming are effective on mouse sperm parameters. Materials and Methods: Adult male mouse(N=35) were randomly divided into 7 groups: 1-controls 2-cold water (100C) with swimming 3- cold w...

BACKGROUND The effectiveness of varicocelectomy in restoration of fertility and improvement of semen parameters is still controversial. The purpose of this study was to determine the effect of age on semen parameters following varicocelectomy in a group of infertile men. METHODS Improvements in sperm count, morphology, and motility were studied in 67 infertile patients 4-10 months after varic...

Semen from 10 932 male partners of infertile couples was analysed and sperm parameter trends were evaluated at the Reproduction Biology Laboratory of the University Hospital of Marseille (France) between 1988 and 2007. After 3-6 days of abstinence, semen samples were collected. Measurements of seminal fluid volume, pH, sperm concentration, total sperm count, motility and detailed morphology of ...

We read with great interest the article recently published by Menkeveld et al. as we have a double interest in sperm morphology assessment. The first one as a Clinical Andrology Laboratory (Laboratory of Male Fertility, Department of Clinical Biochemistry, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Argentina) attempting to give valuable diagnosis to our patients, and the ...

OBJECTIVE To evaluate the immediate effect of hands-on training on the quality of technical skills of laboratory technicians. DESIGN Retrospective analytical study. SETTING Academic institutions and private infertility clinics. PARTICIPANTS One hundred and ten laboratory technicians. INTERVENTION(S) None. MAIN OUTCOME MEASURE(S) First, 110 participants from 16 African countries attend...

OBJECTIVE To study the effect of peritoneal fluid (PF) from healthy women and from women with endometriosis on sperm DNA and its relationship to sperm morphology as assessed by the sperm deformity index (SDI). DESIGN Experimental study. SETTING Research laboratory at an academic hospital. PATIENT(S) Healthy women undergoing laparoscopic tubal ligation and women with endometriosis. INTER...