Background: Following the expansion of human infertility, different procedures have been utilized for separation of normal sperm during ICSI (Intra cytoplasmic sperm injection). Hypo-osmotic swelling test (HOST) is one of the tests which have been introduced for assessment of integrity of sperm membrane when confronted with hypo osmotic condition. This test may also reflect intracellular organe...

Introduction: We aimed to determine pregnancy and miscarriage rates following intracytoplasmic sperm injection (ICSI) cycles using retrieved epididymal and testicular sperm in azoospermic men and ejaculated sperm in oligospermic and normospermic men. Materials and Methods: This retrospective study was carried out on 517 couples who underwent ICSI. They included 96 couples with azoospermia and 4...

PURPOSE We review the history of the cryopreservation of human sperm. The current methods of cryopreservation and indications are highlighted, and the success rates of the various uses of cryopreserved sperm are reviewed. Potential adverse sequelae of human cryopreservation are also discussed. MATERIALS AND METHODS Indications for cryopreservation include several applications, namely homologo...

Background Failure of Male Pronucleus (MPN) formation is a major concern in the success of Intracytoplasmic Sperm Injection (ICSI) in some species. Despite the conducted unsuccessful efforts to improve ICSI efficiency in ovine, the present study was aimed to improve MPN formation and embryo development in ovine ICSI procedure through accompaniment of sperm pretreatment with co-injection of some...

background & objectives : the purpose of this study was to compare the success rate of intracytoplasmic sperm injection cycles (icsi) between infertile war victims & other infertile couples . materials & methods :this retrospective study was done on 672 infertile couples in kossar fertility & impotence center from 2000-2002. poisson, chi-square & t–test were used to compare the icsi results in ...

Sperm are highly differentiated and the activities that reprogram them for embryonic development during fertilization have historically been considered unique to the oocyte. We here challenge this view and demonstrate that mouse embryos in the mitotic cell cycle can also directly reprogram sperm for full-term development. Developmentally incompetent haploid embryos (parthenogenotes) injected wi...

Our study objective was to assess the effect of various sperm DNA fragmentation levels on clinical intracytoplasmic injection outcome. This retrospective included 392 patients who underwent ICSI and performed testing before procedure. Based cut-off values, were differentiated into 3 groups as <20%, 20%–30% >30%. According female status, favourable group (n = 259) with age <35 years anti-Mulleri...

Retraction The Editors-in-Chief are retracting this article [1] because the study was not conducted with the appropriate ethical oversight. Contrary to the statement in the article that ethics committee approval was not required, an investigation by the Arcispedale Santa Maria NuovaIRCCS has determined that the study should have been reviewed and approved by an ethics committee. All authors agr...

The morphology of the sperm head has often been correlated with the outcome of in vitro fertilization, and has been shown to be the sole parameter in semen of value in predicting the success of intracytoplasmic sperm injection and intracytoplasmic morphologically selected sperm injection. In this paper, we have studied whether digital holographic microscopy (DHM) may be useful to obtain quantit...

The FertDish features a clinically applicable sperm processing format, and enables high recovery of motile with DNA quality.