The present study describes development and subsequent validation of high performance liquid chromatographic method (HPLC) in comparison with spectrophotometric methods (classic, first, second and third order derivative) for determination of pure cilazapril in substance and pharmaceutical preparation. The main aim of this study was to find the method suitable not only for determination of cilaz...

An isocratic RP-HPLC method (Method-A) and two visible spectrophotometric methods (Method B and C) were developed and validated for the determination of Clobazam in bulk and tablet dosage forms. In Method-A, a PEAK chromatographic system equipped with Zodiac C-18 (250mm, 4.6mm and 5μm) column, mobile phase of composition 10:30:60 (v/v/v) of tetra hydro furan (THF), methanol (MeOH) and acetonitr...

A simple, sensitive, cost-effective and reproducible UV-spectrophotometric method has been developed and validated for the estimation of disodium edetate in topical gel formulations. Solution of disodium edetate reacts with ferric chloride to form complex in 0.1 N HCl giving λmax at 270 nm. Beer's law was obeyed in the concentration range of 5-50 μg/mL (r (2)= 0.9997). The limit of detection an...

A simple, sensitive, rapid and accurate spectrophotometric method was developed for the determination of risedronate, a bisphosphonate drug important for the treatment of a variety of bone diseases, in raw material and pharmaceutical formulations. The proposed method is based on complex formation between risedronate and Cu (II) ions in acetate buffer of pH5.5. The optimum conditions for this re...

AIM AND OBJECTIVES To develop a simple, sensitive, cost-effective and reproducible UV-spectrophotometric method and validate for the estimation of disodium edetate in topical gel formulations. MATERIALS AND METHODS Solution of disodium edetate reacts with ferric chloride to form complex in 0.1 N HCl giving λmax at 270 nm. Beer's law was obeyed in the concentration range of 5-50 μg/mL (r (2) =...

Objective: To develop a simple and cheap UV spectrophotometric method for the quantitative estimation of Indinavir sulphate (400mg) in capsules and validate as per ICH guidelines. Methods: The optimized method uses a diluent 100% potassium dihydrogen ortho phosphate buffer (pH 4.0) for the estimation of assay of Indinavir sulphate whose λmax is 259 nm. Results: The developed method resulted in ...

A rapid, relatively sensitive, and low-cost method for the determination of water-soluble urea content in dermatological therapy products and cosmetics is proposed using a new spectrophotometric assay with water as the only extraction solvent. Spectrophotometric methods involve addition of a known excess of bromate to urea in an acid medium, followed by the determination of residual bromine and...

Cloud point extraction (CPE) was used for the preconcentration of Hg (II) by spectrophotometric method.In this complex, Triton-X-114,Cetylpyridinium chloride (CPC) and NaCl were applied as the non ionic surfactant, sensitizing agent and electrolyte respectively.The lower limit of detection (LOD) acquired under the optimum conditions was 1.10μg L-1. Therelative standard deviations(RSDs) for ten ...

In the present work simple, precise, specific, accurate and cost effective UV spectrophotometric method has been developed for the determination of Mirabegron in pure and pharmaceutical formulation. The wavelength of the Mirabegron detection was 251 nm using methanol as a solvent and obeys Beer’s law in the concentration range of 0.2 – 1.2 μg /mL with a correlation coefficient of 0.9991. Relati...

This work presents the development of a methodology based on the formation of a charge transfer complex between quinalizarin and rosuvastatin, allowing for the spectrophotometric determination of rosuvastatin at 579 nm. The factors involved in the sensitivity of the technique were studied (nature and proportion of the solvent, reaction time, pH of aqueous phase and quinalizarin concentration). ...