Eighteen hybridoma cell lines were used to study species-specific, stage-specific, and serological cross-reactive antigens of the rodent malarial parasite, Plasmodium yoelii. Specificity and location of plasmodial antigens were determined by indirect fluorescent-antibody analysis. Results showed that a minimum of 12 distinct plasmodial antigens could be distinguished by the 18 hybridomas. Antig...

Hydatid cyst fluid has been used as a source of antigen for the serodiagnosis of Echinococcus granulosus infection. Due to cross-reactions with antigens shared by other helminthes, the specific antigen from hydatid cyst fluid was purified by many workers. We used a relatively simple technique for purification of specific antigen from sheep hydatid cyst fluid. Isopycnic ultracentrifugation w...

visceral leishmaniasis (vl) is a zoonotic disease caused by leishmania species. dogs are considered to be the main reservoir of vl. a number of methods and antigen-based assays are used for the diagnosis of leishmaniasis. however, currently available methods are mainly based on direct examination of tissues for the presence of parasites, which is highly invasive. a variety of serological tests ...

dendritic cells (dcs) are antigen presenting cells with unique capability to take up and process antigens in the peripheral blood and tissues. they subsequently migrate to draining lymph nodes where they present these antigens and stimulate naive t lympho-cytes. during their life cycle, dcs go through two maturation stages and are referred to as immature and mature cells, respectively. while im...

Background A specific feature of the autoimmune response in rheumatoid arthritis (RA) is presence anti-citrullinated protein antibodies (ACPAs) patient sera. These can appear several years before disease onset and are involved development RA. Objective We performed proteomic analysis by mass spectroscopy to identify novel citrullinated antigens autoantibodies RA patients. Methods Polypeptides i...

While first and foremost considered a respiratory infection, COVID-19 can result in complications affecting multiple organs. Immune responses both protect against the disease as well drive it. Insights into these responses, specifically targets being recognised by immune system, are of vital importance understanding side effects associated pathologies. The body’s adaptive immunity recognises re...

introduction: as monoclonal antibodies are potential tools for characterization of soluble or cellular surface antigens, use of these proteins has always been considered in infertility and reproduction research. therefore, in this study, monoclonal antibodies against human sperm surface antigens were produced. material and methods: to produce specific clones against human sperm surface antigens...

ABSTRACT: The aims of this study were: 1) determine total specific IgG and subisotypes in serum colostrum pregnant mares vaccinated against Theileria equi Streptococcus subspecies equi; 2) foals born from these mares. In mares, the highest value for T. was at 335 days gestation declining 30 postpartum, while S. 328 remaining high up to postpartum. Transfer passive immunity both antigens observe...

  Escherichia coli O157:H7 is an important human pathogen causing haemorrhagic colitis, haemolyticuraemic syndrom and thrombotic thrombocytopenic purpura. In this study, 100 ground beef samples were collected randomly from beef markets in June 2004. For isolation of the bacteria, samples were firstly enriched in modified trypticase soy broth, followed by plating onto sorbitol MacConkey agar sup...