In this paper we report on the evidence for ionization track signals from cosmic ray muons and Compton electrons in a Time Projection Chamber (TPC) filled with liquid Argon and doped with different fractions of Nitrogen. This study has been conducted in view of the possible use of liquid Argon/Nitrogen TPCs for the detection of gamma rays in the resonant band of the Nitrogen absorbtion spectrum...

In this chapter we discuss the latest developments in the field of high-pressure freezing (HPF). The Leica HPF machine EM HPM100 is discussed in detail due to significant changes compared to its predecessor model. Its centerpiece is a multipart polymer cartridge which holds the specimen carrier sandwich and guides it automatically through the freezing process until immersed in liquid nitrogen. ...

Mechanism of Action Liquid nitrogen, which boils at −196°C (−320.8°F), is the most effective cryogen for clinical use. It is particularly useful in the treatment of malignant lesions. Temperatures of −25°C to −50°C (−13°F to −58°F) can be achieved within 30 seconds if a sufficient amount of liquid nitrogen is applied by spray or probe. Generally, destruction of benign lesions requires temperatu...

Mammalian cells including human cancer cells are usually transported in cryovials on dry ice or in a liquid nitrogen vapor shipping vessel between different places at long distance. The hazardous nature of dry ice and liquid nitrogen, and the associated high shipping cost strongly limit their routine use. In this study, we tested the viability and properties of cells after being preserved or sh...

Nitrogen-filled “Vacutainers” (Becton-Dickinson, Rutherford, N. J. 07070) have been proposed for the anaerobic collection of specimens for blood gas determinations (1). We have indicated (2) that these devices, as currently supplied by the manufacturer, contain oxygen and may produce spuriously high P(02) and percentage oxyhemogobin (HbO) values. We had previously indicated preference for the u...

The isolation of intact, high-molecular mass genomic DNA is essential for many molecular biology applications including PCR, endonuclease digestion, southern blot analysis, and genomic library construction. Many protocols are available for the extraction of genomic DNA from plant tissue, and all the protocols have used expensive and hazardous chemical-liquid nitrogen for grinding plant material...