somatic embryos of persian walnut were transformed with two strains of agrobacterium tumefaciens i.e. lba4404 and c58, and two plasmids, namely, pbi121 with nptii and gus genes for improving the transformation protocol, and p6u-ubi-fvti plasmid containing the hpt and fld genes. the transformation frequency was 10%. pcr and rt-pcr analysis proved the presence and expression of the genes. the tra...

germline and somatic stem cells are distinct types of stem cells that are dedicated to reproduction and somatic tissue regeneration, respectively. germline stem cells (gscs), which can self-renew and generate gametes, are unique stem cells in that they are solely dedicated to transmit genetic information from generation to generation. we developed a strategy for the establishment of germline st...

the aim of this study was to estimate the genetic and phonotypic trends of somatic cell score in iranian holstein cows. also, the environmental factors affecting this trait are investigated. a total of 375351 test day records of somatic cells take from 54374 animals between year 2002 and 2007 were used in this study. the breeding values were estimated by fitting animal model in asreml software....

Real time-qPCR is the most reliable method for evaluation of mRNA expression levels. However, to obtain accurate results, selection of suitable reference genes is necessary for normalizing the real-time qPCR data. The aim of this research was to validate the expression stability of three potential reference genes (ACTB, GAPDH and UXT) in milk somatic cells of Holstein dairy cattle under differe...

Objective: The present study was done to investigate the mediating role of cognitive emotion regulation strategies in the relationship between early maladaptive schemas, alexithymia, and emotional intelligence with the somatic symptoms in people with somatic symptom disorder (SSD). Methods: The research population consisted of 360 people with SSD referred to the psychosomatic department of Tal...